Mak DO, McBride S, Foskett JK.

GM56328 NIGMS NIH HHS , MH59937 NIMH NIH HHS, R37 GM056328 NIGMS NIH HHS , R01 MH059937 NIMH NIH HHS, R01 GM056328 NIGMS NIH HHS

	Figure 1. Expression of endogenous X-InsP3R-1 and recombinant r-InsP3R-3 in cRNA-injected (+) and control, uninjected (−) Xenopus oocytes. Western analysis was performed as described in Mak et al. 2000. (A–H) Immunoblotted with InsP3R-1-specific antibody (Joseph and Samanta 1993; Joseph et al. 1995); (I–J) immunoblotted with InsP3R-3-specific antibody (Transduction Labs.). Aliquots equivalent to n oocytes from the same lysate sample were used in the lanes.
	Figure 2. Typical single-channel current traces of the r-InsP3R-3 at various [Ca2+]i in the presence of 10 μM InsP3. Arrows indicate closed channel current level in all current traces.
	Figure 3. Ca2+ dependencies of mean open channel duration (Α) and closed channel duration (B) of the r-InsP3R-3 activated by various concentrations of InsP3 as tabulated. In the closed channel duration graph, data points obtained with the same InsP3 concentration are connected with a line for clarity.
	Figure 4. Ca2+ dependence of r-InsP3R-3 channel open probability under various [InsP3]. Different symbols denote data for various [InsP3] as tabulated. The curves are theoretical fits using the Hill equation (), with Kinh varying with [InsP3] as listed in the graph, whereas Pmax, Kact, Hact, and Hinh remained independent of [InsP3] with values tabulated in the graph.
	Figure 5. Single-channel open and closed channel duration histograms of r-InsP3R-3 in 10 μM InsP3, with 29 nM, 224 nM, 1.2 μM, and 57.5 μM Ca2+, respectively, as listed in the graphs. The open and closed duration histograms shown for each [Ca2+]i were derived from one patch-clamp experiment performed under the stated [Ca2+]i. Similar histograms were obtained from two additional experiments at each set of experimental conditions. The smooth curves are theoretical probability density functions consisting of two to four exponential components fitted to the histograms, as outlined in Sigworth and Sine 1987. The time constant and relative weight of each exponential component is labeled besides the corresponding peak in the curves. Duration histograms obtained with the same set of experimental conditions are fitted with the same number of exponential components and the time constants and relative weights of corresponding exponential components lie within ∼30% of the values shown.
	Figure 6. Comparison of the Ca2+ dependencies of Po of r-InsP3R-3 and X-InsP3R-1 in 10 μM InsP3 and 0.5 mM ATP. Open circles represent data for r-InsP3R-3 from this study, fitted with the solid curve; closed circles represent data for X-InsP3R-1 taken from (Mak et al. 1998), fitted with the dashed curve. The curves are calculated using the Hill equation () with the tabulated parameters. Higher affinity and lack of cooperativity of the Ca2+ activation sites of the type 3 channel endow it with high gain IICR and low gain CICR. In contrast, lower affinity and presence of cooperativity of the Ca2+ activation sites of the type 1 channel confer low gain IICR and high gain CICR. Under resting [Ca2+]i, low levels of stimulation will trigger release of Ca2+ by IICR from the type 3 channel, which in turn will trigger further release by CICR from the type 1 channel.
	Figure 7. Single-channel open and closed channel duration histograms and the fitted theoretical probability density functions of X-InsP3R-1 in 10 μM InsP3 with 31 and 224 nM Ca2+, respectively, obtained as described in the legend to Fig. 5.

Amundson, Calcium waves. 1993, Pubmed, Xenbase

Amundson, Calcium waves. 1993, Pubmed , Xenbase
Atri, A single-pool model for intracellular calcium oscillations and waves in the Xenopus laevis oocyte. 1993, Pubmed , Xenbase
Baudet, How to make and use calcium-specific mini- and microelectrodes. 1994, Pubmed
Benevolensky, Micromolar calcium decreases affinity of inositol trisphosphate receptor in vascular smooth muscle. 1994, Pubmed
Berridge, Inositol trisphosphate and calcium signalling. 1993, Pubmed
Bezprozvanny, Caffeine-induced inhibition of inositol(1,4,5)-trisphosphate-gated calcium channels from cerebellum. 1994, Pubmed
Bezprozvanny, Inositol (1,4,5)-trisphosphate (InsP3)-gated Ca channels from cerebellum: conduction properties for divalent cations and regulation by intraluminal calcium. 1994, Pubmed
Bezprozvanny, Bell-shaped calcium-response curves of Ins(1,4,5)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum. 1991, Pubmed
Bezprozvanny, The inositol 1,4,5-trisphosphate (InsP3) receptor. 1995, Pubmed
Blondel, Sequence and functional characterization of a third inositol trisphosphate receptor subtype, IP3R-3, expressed in pancreatic islets, kidney, gastrointestinal tract, and other tissues. 1993, Pubmed
Boehning, Functional properties of recombinant type I and type III inositol 1, 4,5-trisphosphate receptor isoforms expressed in COS-7 cells. 2000, Pubmed
Boitano, Intercellular propagation of calcium waves mediated by inositol trisphosphate. 1992, Pubmed
Bootman, The elemental principles of calcium signaling. 1995, Pubmed
Bush, Epithelial inositol 1,4,5-trisphosphate receptors. Multiplicity of localization, solubility, and isoforms. 1994, Pubmed
Cardy, Differential regulation of types-1 and -3 inositol trisphosphate receptors by cytosolic Ca2+. 1997, Pubmed
Carter, Kinetics of Ca2+ release by InsP3 in pig single aortic endothelial cells: evidence for an inhibitory role of cytosolic Ca2+ in regulating hormonally evoked Ca2+ spikes. 1997, Pubmed
Clapham, Calcium signaling. 1995, Pubmed
Danoff, Inositol 1,4,5-trisphosphate receptors: distinct neuronal and nonneuronal forms derived by alternative splicing differ in phosphorylation. 1991, Pubmed
De Smedt, Determination of relative amounts of inositol trisphosphate receptor mRNA isoforms by ratio polymerase chain reaction. 1994, Pubmed
De Smedt, Isoform diversity of the inositol trisphosphate receptor in cell types of mouse origin. 1997, Pubmed
Dufour, Inositol 1,4,5-trisphosphate and calcium regulate the calcium channel function of the hepatic inositol 1,4,5-trisphosphate receptor. 1997, Pubmed
Ferris, Inositol phosphate receptors and calcium disposition in the brain. 1992, Pubmed
Fujino, Differential expression of type 2 and type 3 inositol 1,4,5-trisphosphate receptor mRNAs in various mouse tissues: in situ hybridization study. 1995, Pubmed
Furuichi, Intracellular channels. 1994, Pubmed
Furuichi, Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400. 1989, Pubmed
Furuichi, Inositol 1, 4, 5-trisphosphate receptor-mediated Ca2+ signaling in the brain. 1995, Pubmed
Hagar, Regulation of the type III InsP(3) receptor by InsP(3) and ATP. 2000, Pubmed
Hagar, Type III InsP3 receptor channel stays open in the presence of increased calcium. 1998, Pubmed
Hingorani, Assay and purification of neuronal receptors for inositol 1,4,5-trisphosphate. 1992, Pubmed
Hirota, Calmodulin inhibits inositol 1,4,5-trisphosphate-induced calcium release through the purified and reconstituted inositol 1,4,5-trisphosphate receptor type 1. 1999, Pubmed
Honda, Gq pathway desensitizes chemotactic receptor-induced calcium signaling via inositol trisphosphate receptor down-regulation. 1995, Pubmed , Xenbase
Iino, Feedback control of inositol trisphosphate signalling bycalcium. 1994, Pubmed
Jiang, Cystic fibrosis transmembrane conductance regulator-associated ATP release is controlled by a chloride sensor. 1998, Pubmed , Xenbase
Joseph, Heteroligomers of type-I and type-III inositol trisphosphate receptors in WB rat liver epithelial cells. 1995, Pubmed
Joseph, The inositol triphosphate receptor family. 1996, Pubmed
Joseph, Factors determining the composition of inositol trisphosphate receptor hetero-oligomers expressed in COS cells. 2000, Pubmed
Joseph, Detergent solubility of the inositol trisphosphate receptor in rat brain membranes. Evidence for association of the receptor with ankyrin. 1993, Pubmed
Kaftan, Inositol 1,4,5-trisphosphate (InsP3) and calcium interact to increase the dynamic range of InsP3 receptor-dependent calcium signaling. 1997, Pubmed
Kasai, Spatial dynamics of second messengers: IP3 and cAMP as long-range and associative messengers. 1994, Pubmed
Kume, The Xenopus IP3 receptor: structure, function, and localization in oocytes and eggs. 1993, Pubmed , Xenbase
Lechleiter, Molecular mechanisms of intracellular calcium excitability in X. laevis oocytes. 1992, Pubmed , Xenbase
Lee, Polarized expression of Ca2+ channels in pancreatic and salivary gland cells. Correlation with initiation and propagation of [Ca2+]i waves. 1997, Pubmed
Lupu, Functional coupling of phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate receptor. 1998, Pubmed
Maeda, Structural and functional characterization of inositol 1,4,5-trisphosphate receptor channel from mouse cerebellum. 1991, Pubmed
Magnusson, Calcium-induced degradation of the inositol (1,4,5)-trisphosphate receptor/Ca(2+)-channel. 1993, Pubmed
Mak, ATP regulation of type 1 inositol 1,4,5-trisphosphate receptor channel gating by allosteric tuning of Ca(2+) activation. 1999, Pubmed , Xenbase
Mak, Single-channel kinetics, inactivation, and spatial distribution of inositol trisphosphate (IP3) receptors in Xenopus oocyte nucleus. 1997, Pubmed , Xenbase
Mak, Inositol 1,4,5-trisphosphate [correction of tris-phosphate] activation of inositol trisphosphate [correction of tris-phosphate] receptor Ca2+ channel by ligand tuning of Ca2+ inhibition. 1998, Pubmed , Xenbase
Mak, Single-channel inositol 1,4,5-trisphosphate receptor currents revealed by patch clamp of isolated Xenopus oocyte nuclei. 1994, Pubmed , Xenbase
Mak, Effects of divalent cations on single-channel conduction properties of Xenopus IP3 receptor. 1998, Pubmed , Xenbase
Mak, Single-channel properties in endoplasmic reticulum membrane of recombinant type 3 inositol trisphosphate receptor. 2000, Pubmed , Xenbase
Maranto, Primary structure, ligand binding, and localization of the human type 3 inositol 1,4,5-trisphosphate receptor expressed in intestinal epithelium. 1994, Pubmed
Marshall, Two calcium-binding sites mediate the interconversion of liver inositol 1,4,5-trisphosphate receptors between three conformational states. 1994, Pubmed
Mauger, The inositol 1,4,5-trisphosphate receptor: kinetic properties and regulation. 1994, Pubmed
Meyer, Highly cooperative opening of calcium channels by inositol 1,4,5-trisphosphate. 1988, Pubmed
Michikawa, Calmodulin mediates calcium-dependent inactivation of the cerebellar type 1 inositol 1,4,5-trisphosphate receptor. 1999, Pubmed
Mignery, Putative receptor for inositol 1,4,5-trisphosphate similar to ryanodine receptor. 1989, Pubmed
Missiaen, Functional properties of the type-3 InsP3 receptor in 16HBE14o- bronchial mucosal cells. 1998, Pubmed
Missiaen, Calmodulin increases the sensitivity of type 3 inositol-1,4, 5-trisphosphate receptors to Ca(2+) inhibition in human bronchial mucosal cells. 2000, Pubmed
Miyakawa, Encoding of Ca2+ signals by differential expression of IP3 receptor subtypes. 1999, Pubmed
Monkawa, Localization of inositol 1,4,5-trisphosphate receptors in the rat kidney. 1998, Pubmed
Monkawa, Heterotetrameric complex formation of inositol 1,4,5-trisphosphate receptor subunits. 1995, Pubmed
Nakagawa, The subtypes of the mouse inositol 1,4,5-trisphosphate receptor are expressed in a tissue-specific and developmentally specific manner. 1991, Pubmed
Newton, Co-expression in vertebrate tissues and cell lines of multiple inositol 1,4,5-trisphosphate (InsP3) receptors with distinct affinities for InsP3. 1994, Pubmed
Nucifora, Inositol 1,4,5-trisphosphate receptors in endocrine cells: localization and association in hetero- and homotetramers. 1996, Pubmed
Parker, Elementary events of InsP3-induced Ca2+ liberation in Xenopus oocytes: hot spots, puffs and blips. 1996, Pubmed , Xenbase
Patel, Ca2+-independent inhibition of inositol trisphosphate receptors by calmodulin: redistribution of calmodulin as a possible means of regulating Ca2+ mobilization. 1997, Pubmed
Perez, Identification and functional reconstitution of the type 2 inositol 1,4,5-trisphosphate receptor from ventricular cardiac myocytes. 1997, Pubmed
Putney, The inositol phosphate-calcium signaling system in nonexcitable cells. 1993, Pubmed
Ramos-Franco, Isoform-specific function of single inositol 1,4,5-trisphosphate receptor channels. 1998, Pubmed
Rooney, Intracellular calcium waves generated by Ins(1,4,5)P3-dependent mechanisms. 1993, Pubmed
Schrenzel, Highly cooperative Ca2+ elevations in response to Ins(1,4,5)P3 microperfusion through a patch-clamp pipette. 1995, Pubmed
Sigworth, Data transformations for improved display and fitting of single-channel dwell time histograms. 1987, Pubmed
Stehno-Bittel, Calcium release from the nucleus by InsP3 receptor channels. 1995, Pubmed , Xenbase
Südhof, Structure of a novel InsP3 receptor. 1991, Pubmed
Sugiyama, Monoclonal antibodies distinctively recognizing the subtypes of inositol 1,4,5-trisphosphate receptor: application to the studies on inflammatory cells. 1994, Pubmed
Supattapone, Solubilization, purification, and characterization of an inositol trisphosphate receptor. 1988, Pubmed
Swatton, Type 3 inositol trisphosphate receptors in RINm5F cells are biphasically regulated by cytosolic Ca2+ and mediate quantal Ca2+ mobilization. 1999, Pubmed
Taylor, Calcium and inositol 1,4,5-trisphosphate receptors: a complex relationship. 1992, Pubmed
Taylor, Calcium and inositol trisphosphate receptors. 1995, Pubmed
Taylor, Structure and function of inositol trisphosphate receptors. 1991, Pubmed
Toescu, Temporal and spatial heterogeneities of Ca2+ signaling: mechanisms and physiological roles. 1995, Pubmed
Watras, Inositol 1,4,5-trisphosphate-gated channels in cerebellum: presence of multiple conductance states. 1991, Pubmed
Welch, Structural components of ryanodine responsible for modulation of sarcoplasmic reticulum calcium channel function. 1997, Pubmed
Wojcikiewicz, Type I, II and III inositol 1,4,5-trisphosphate receptor co-immunoprecipitation as evidence for the existence of heterotetrameric receptor complexes. 1995, Pubmed
Wojcikiewicz, Type I, II, and III inositol 1,4,5-trisphosphate receptors are unequally susceptible to down-regulation and are expressed in markedly different proportions in different cell types. 1995, Pubmed
Wojcikiewicz, Muscarinic receptor activation down-regulates the type I inositol 1,4,5-trisphosphate receptor by accelerating its degradation. 1994, Pubmed
Yamada, The calmodulin-binding domain in the mouse type 1 inositol 1,4,5-trisphosphate receptor. 1995, Pubmed
Yoneshima, Ca2+ differentially regulates the ligand-affinity states of type 1 and type 3 inositol 1,4,5-trisphosphate receptors. 1997, Pubmed
Yule, Evidence that zymogen granules are not a physiologically relevant calcium pool. Defining the distribution of inositol 1,4,5-trisphosphate receptors in pancreatic acinar cells. 1997, Pubmed