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Proc Natl Acad Sci U S A
2001 Jul 03;9814:8133-8. doi: 10.1073/pnas.131186798.
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The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae.
Voegele RT, Struck C, Hahn M, Mendgen K.
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Biotrophic plant pathogenic fungi differentiate specialized infection structures within the living cells of their host plants. These haustoria have been linked to nutrient uptake ever since their discovery. We have for the first time to our knowledge shown that the flow of sugars from the host Vicia faba to the rust fungus Uromyces fabae seems to occur largely through the haustorial complex. One of the most abundantly expressed genes in rust haustoria, the expression of which is negligible in other fungal structures, codes for a hexose transporter. Functional expression of the gene termed HXT1 in Saccharomyces cerevisiae and Xenopus laevis oocytes assigned a substrate specificity for D-glucose and D-fructose and indicated a proton symport mechanism. Abs against HXT1p exclusively labeled haustoria in immunofluorescence microscopy and the haustorial plasma membrane in electron microscopy. These results suggest that the fungus concentrates this transporter in haustoria to take advantage of a specialized compartment of the haustorial complex. The extrahaustorial matrix, delimited by the plasma membranes of both host and parasite, constitutes a newly formed apoplastic compartment with qualities distinct from those of the bulk apoplast. This organization might facilitate the competition of the parasite with natural sink organs of the host.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Elble,
A simple and efficient procedure for transformation of yeasts.
1992,
Pubmed Hahn,
A putative amino acid transporter is specifically expressed in haustoria of the rust fungus Uromyces fabae.
1997,
Pubmed Hahn,
Characterization of in planta-induced rust genes isolated from a haustorium-specific cDNA library.
1997,
Pubmed Hasegawa,
Rodent polyphyly?
1992,
Pubmed Hillis,
Hobgoblin of phylogenetics?
1994,
Pubmed Marger,
A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport.
1993,
Pubmed Mendgen,
The activity of powdery-mildew haustoria after feeding the host cells with different sugars, as measured with a potentiometric cyanine dye.
1988,
Pubmed Nehls,
Carbon allocation in ectomycorrhizas: identification and expression analysis of an Amanita muscaria monosaccharide transporter.
1998,
Pubmed NULL,
Guide to yeast genetics and molecular biology.
1991,
Pubmed Ozcan,
Function and regulation of yeast hexose transporters.
1999,
Pubmed Philippe,
MUST, a computer package of Management Utilities for Sequences and Trees.
1993,
Pubmed Reifenberger,
Kinetic characterization of individual hexose transporters of Saccharomyces cerevisiae and their relation to the triggering mechanisms of glucose repression.
1997,
Pubmed Reifenberger,
Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux.
1995,
Pubmed Rentsch,
NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
1995,
Pubmed Seligman,
A new staining method (OTO) for enhancing contrast of lipid--containing membranes and droplets in osmium tetroxide--fixed tissue with osmiophilic thiocarbohydrazide(TCH).
1966,
Pubmed Strimmer,
Likelihood-mapping: a simple method to visualize phylogenetic content of a sequence alignment.
1997,
Pubmed Struck,
Plasma Membrane H+-ATPase Activity in Spores, Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae.
1996,
Pubmed Struck,
The plasma membrane H(+)-ATPase from the biotrophic rust fungus Uromyces fabae: molecular characterization of the gene (PMA1) and functional expression of the enzyme in yeast.
1998,
Pubmed Studier,
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
1986,
Pubmed Szabo,
Hidden robbers: the role of fungal haustoria in parasitism of plants.
2001,
Pubmed Van de Peer,
Construction of evolutionary distance trees with TREECON for Windows: accounting for variation in nucleotide substitution rate among sites.
1997,
Pubmed
