Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2001 Jul 31;9816:9431-6. doi: 10.1073/pnas.161099898.
Show Gene links
Show Anatomy links
Expression cloning of two genes that together mediate organic solute and steroid transport in the liver of a marine vertebrate.
Wang W, Seward DJ, Li L, Boyer JL, Ballatori N.
???displayArticle.abstract???
Uptake of organic solutes and xenobiotics by mammalian cells is mediated by ATP-independent transporters, and four families of transporters have now been identified. To search for novel organic solute transporters, a liver cDNA library from an evolutionarily primitive marine vertebrate, the little skate Raja erinacea, was screened for taurocholate transport activity by using Xenopus laevis oocytes. In contrast to the organic anion transporters identified to date, a transport activity was identified in this library that required the coexpression of two distinct gene products, termed organic solute transporter alpha and beta (Ostalpha, Ostbeta). Ostalpha cDNA encodes for a protein of 352 aa and seven putative transmembrane (TM) domains. Ostbeta contains 182 aa and has at least one and perhaps two TM domains. There is no significant sequence identity between Ostalpha and Ostbeta, and only low identity with sequences in the databases; however, Ostalpha bears a resemblance to some G protein-coupled receptors, and Ostbeta exhibits 22% amino acid identity with the C-terminal TM and intracellular domains of protocadherin-gamma, a cell surface glycoprotein. Xenopus oocytes injected with the cRNA for both Ostalpha and Ostbeta, but not each separately, were able to take up taurocholate, estrone sulfate, digoxin, and prostaglandin E(2), but not p-aminohippurate or S-dinitrophenyl glutathione. Transport was sodium-independent, saturable, and inhibited by organic anions and steroids, including the major skate bile salt, scymnol sulfate. These results identify an organic anion transporter composed of a putative seven-helix TM protein and an ancillary membrane polypeptide.
Angst,
The cadherin superfamily: diversity in form and function.
2001, Pubmed
Angst,
The cadherin superfamily: diversity in form and function.
2001,
Pubmed Ballatori,
Multiple canalicular transport mechanisms for glutathione S-conjugates. Transport on both ATP- and voltage-dependent carriers.
1995,
Pubmed Ballatori,
Bile salt excretion in skate liver is mediated by a functional analog of Bsep/Spgp, the bile salt export pump.
2000,
Pubmed Ballatori,
An endogenous ATP-sensitive glutathione S-conjugate efflux mechanism in Xenopus laevis oocytes.
1996,
Pubmed
,
Xenbase Bertran,
Stimulation of system y(+)-like amino acid transport by the heavy chain of human 4F2 surface antigen in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase Blanco,
Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function.
1998,
Pubmed Borst,
A family of drug transporters: the multidrug resistance-associated proteins.
2000,
Pubmed Boyer,
Phylogenic and ontogenic expression of hepatocellular bile acid transport.
1993,
Pubmed
,
Xenbase Boyer,
Biliary secretion in elasmobranchs. II. Hepatic uptake and biliary excretion of organic anions.
1976,
Pubmed Cai,
Bile salt export pump is highly conserved during vertebrate evolution and its expression is inhibited by PFIC type II mutations.
2001,
Pubmed Chang,
Removal of multiple arginine-framed trafficking signals overcomes misprocessing of delta F508 CFTR present in most patients with cystic fibrosis.
1999,
Pubmed Ellgaard,
Setting the standards: quality control in the secretory pathway.
1999,
Pubmed Fambrough,
Analysis of subunit assembly of the Na-K-ATPase.
1994,
Pubmed Fricker,
Enterohepatic circulation of scymnol sulfate in an elasmobranch, the little skate (Raja erinacea).
1997,
Pubmed Fricker,
Characteristics of bile salt uptake into skate hepatocytes.
1994,
Pubmed Gershengorn,
Minireview: Insights into G protein-coupled receptor function using molecular models.
2001,
Pubmed Goldin,
Maintenance of Xenopus laevis and oocyte injection.
1992,
Pubmed
,
Xenbase Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase Hagenbuch,
Expression of the hepatocyte Na+/bile acid cotransporter in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase Ihara,
Evolution of the archaeal rhodopsins: evolution rate changes by gene duplication and functional differentiation.
1999,
Pubmed Karlaganis,
A bile alcohol sulfate as a major component in the bile of the small skate (Raja erinacea).
1989,
Pubmed Keppler,
Hepatic secretion of conjugated drugs and endogenous substances.
2000,
Pubmed Kirk,
CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression.
2000,
Pubmed Kullak-Ublick,
Hepatic transport of bile salts.
2000,
Pubmed Kunzelmann,
CFTR, a regulator of channels.
1999,
Pubmed Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed Li,
Identification of glutathione as a driving force and leukotriene C4 as a substrate for oatp1, the hepatic sinusoidal organic solute transporter.
1998,
Pubmed
,
Xenbase Li,
Oatp2 mediates bidirectional organic solute transport: a role for intracellular glutathione.
2000,
Pubmed
,
Xenbase Margeta-Mitrovic,
A trafficking checkpoint controls GABA(B) receptor heterodimerization.
2000,
Pubmed
,
Xenbase Milligan,
Neurobiology. Receptors as kissing cousins.
2000,
Pubmed Naren,
Syntaxin 1A inhibits CFTR chloride channels by means of domain-specific protein-protein interactions.
1998,
Pubmed
,
Xenbase Palacín,
Molecular biology of mammalian plasma membrane amino acid transporters.
1998,
Pubmed Peters,
Syntaxin 1A inhibits regulated CFTR trafficking in xenopus oocytes.
1999,
Pubmed
,
Xenbase Rebbeor,
ATP-dependent GSH and glutathione S-conjugate transport in skate liver: role of an Mrp functional homologue.
2000,
Pubmed Rocheville,
Receptors for dopamine and somatostatin: formation of hetero-oligomers with enhanced functional activity.
2000,
Pubmed Saier,
A functional-phylogenetic classification system for transmembrane solute transporters.
2000,
Pubmed Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase Smith,
Taurocholate uptake by isolated skate hepatocytes: effect of albumin.
1987,
Pubmed Snutch,
Tissue RNA as source of ion channels and receptors.
1992,
Pubmed
,
Xenbase Soreq,
Xenopus oocyte microinjection: from gene to protein.
1992,
Pubmed
,
Xenbase Spudich,
Structural biology. A chloride pump at atomic resolution.
2000,
Pubmed Suzuki,
Transport of drugs across the hepatic sinusoidal membrane: sinusoidal drug influx and efflux in the liver.
2000,
Pubmed Verrey,
New glycoprotein-associated amino acid transporters.
1999,
Pubmed Wells,
The 4F2 antigen heavy chain induces uptake of neutral and dibasic amino acids in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Wu,
Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes.
2000,
Pubmed Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
