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Connexin29 is uniquely distributed within myelinating glial cells of the central and peripheral nervous systems.
Altevogt BM, Kleopa KA, Postma FR, Scherer SS, Paul DL.
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Although both Schwann cells and oligodendrocytes express connexin32 (Cx32), the loss of this connexin causes demyelination only in the PNS. To determine whether oligodendrocytes might express another connexin that can function in place of Cx32, we searched for novel CNS-specific connexins using reverse transcriptase-PCR and degenerate primers. We identified Cx29, whose transcript was restricted to brain, spinal cord, and sciatic nerve. Developmental expression of Cx29 mRNA in the CNS paralleled that of other myelin-related mRNAs, including Cx32. In the CNS, Cx29 antibodies labeled the internodal and juxtaparanodal regions of small myelin sheaths, whereas Cx32 staining was restricted to large myelinated fibers. In the PNS, Cx29 expression preceded that of Cx32 and declined to lower levels than Cx32 in adulthood. In adult sciatic nerve, Cx29 was primarily localized to the innermost aspects of the myelin sheath, the paranode, the juxtaparanode, and the inner mesaxon. Cx29 displayed a striking coincidence with Kv1.2 K(+) channels, which are localized in the axonal membrane. Both Cx29 and Cx32 were found in the incisures. Cx29 expressed in N2A cells did not induce intercellular conductances but did participate in the formation of active channels when coexpressed with Cx32. Together, these data show that Cx29 and Cx32 are expressed by myelinating glial cells with distinct distributions.
Anzini,
Structural abnormalities and deficient maintenance of peripheral nerve myelin in mice lacking the gap junction protein connexin 32.
1997, Pubmed
Anzini,
Structural abnormalities and deficient maintenance of peripheral nerve myelin in mice lacking the gap junction protein connexin 32.
1997,
Pubmed Arroyo,
Internodal specializations of myelinated axons in the central nervous system.
2001,
Pubmed Arroyo,
On the molecular architecture of myelinated fibers.
2000,
Pubmed Baba,
Completion of myelin compaction, but not the attachment of oligodendroglial processes triggers K(+) channel clustering.
1999,
Pubmed Balice-Gordon,
Functional gap junctions in the schwann cell myelin sheath.
1998,
Pubmed Bergoffen,
Connexin mutations in X-linked Charcot-Marie-Tooth disease.
1993,
Pubmed Browne,
Episodic ataxia/myokymia syndrome is associated with point mutations in the human potassium channel gene, KCNA1.
1994,
Pubmed Bunge,
Glial cells and the central myelin sheath.
1968,
Pubmed Butt,
Oligodendrocytes and the control of myelination in vivo: new insights from the rat anterior medullary velum.
2000,
Pubmed Butt,
Visualization of oligodendrocytes and astrocytes in the intact rat optic nerve by intracellular injection of lucifer yellow and horseradish peroxidase.
1989,
Pubmed Chandross,
Altered connexin expression after peripheral nerve injury.
1996,
Pubmed Chiu,
Functions and distribution of voltage-gated sodium and potassium channels in mammalian Schwann cells.
1991,
Pubmed Dermietzel,
Oligodendrocytes express gap junction proteins connexin32 and connexin45.
1997,
Pubmed Dermietzel,
Differential expression of three gap junction proteins in developing and mature brain tissues.
1989,
Pubmed Duncan,
Abnormal compact myelin in the myelin-deficient rat: absence of proteolipid protein correlates with a defect in the intraperiod line.
1987,
Pubmed Einheber,
The axonal membrane protein Caspr, a homologue of neurexin IV, is a component of the septate-like paranodal junctions that assemble during myelination.
1997,
Pubmed Gabriels,
Connexin43 is highly localized to sites of disturbed flow in rat aortic endothelium but connexin37 and connexin40 are more uniformly distributed.
1998,
Pubmed Haefliger,
Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.
1992,
Pubmed Hertzberg,
A detergent-independent procedure for the isolation of gap junctions from rat liver.
1984,
Pubmed Jiang,
Molecular cloning and functional characterization of chick lens fiber connexin 45.6.
1994,
Pubmed
,
Xenbase Kleopa,
Cellular mechanisms of connexin32 mutations associated with CNS manifestations.
2002,
Pubmed Konishi,
Voltage-gated potassium currents in myelinating Schwann cells in the mouse.
1990,
Pubmed Kunzelmann,
Late onset and increasing expression of the gap junction protein connexin30 in adult murine brain and long-term cultured astrocytes.
1999,
Pubmed Kunzelmann,
Coexpression of connexin45 and -32 in oligodendrocytes of rat brain.
1997,
Pubmed Lemke,
Isolation and sequence of a cDNA encoding the major structural protein of peripheral myelin.
1985,
Pubmed Li,
Connexin32 in oligodendrocytes and association with myelinated fibers in mouse and rat brain.
1997,
Pubmed Mambetisaeva,
Multiple connexin expression in peripheral nerve, Schwann cells, and Schwannoma cells.
1999,
Pubmed Massa,
Cell junctions and intramembrane particles of astrocytes and oligodendrocytes: a freeze-fracture study.
1982,
Pubmed Mi,
Differential distribution of closely related potassium channels in rat Schwann cells.
1995,
Pubmed Milner,
Nucleotide sequences of two mRNAs for rat brain myelin proteolipid protein.
1985,
Pubmed Mullen,
NeuN, a neuronal specific nuclear protein in vertebrates.
1992,
Pubmed
,
Xenbase Nagaoka,
Differential expression of gap junction proteins connexin26, 32, and 43 in normal and crush-injured rat sciatic nerves. Close relationship between connexin43 and occludin in the perineurium.
1999,
Pubmed Nagy,
Connexin26 in adult rodent central nervous system: demonstration at astrocytic gap junctions and colocalization with connexin30 and connexin43.
2001,
Pubmed Nagy,
Connexin30 in rodent, cat and human brain: selective expression in gray matter astrocytes, co-localization with connexin43 at gap junctions and late developmental appearance.
1999,
Pubmed Nagy,
Evidence for the co-localization of another connexin with connexin-43 at astrocytic gap junctions in rat brain.
1997,
Pubmed Neuberg,
Altered molecular architecture of peripheral nerves in mice lacking the peripheral myelin protein 22 or connexin32.
1999,
Pubmed Oh,
Molecular determinants of electrical rectification of single channel conductance in gap junctions formed by connexins 26 and 32.
1999,
Pubmed Pastor,
Dye coupling between spinal cord oligodendrocytes: differences in coupling efficiency between gray and white matter.
1998,
Pubmed Paul,
Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes.
1991,
Pubmed
,
Xenbase Paul,
Molecular cloning of cDNA for rat liver gap junction protein.
1986,
Pubmed Poliak,
Caspr2, a new member of the neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels.
1999,
Pubmed Radeke,
Gene transfer and molecular cloning of the rat nerve growth factor receptor.
,
Pubmed Rash,
Cell-specific expression of connexins and evidence of restricted gap junctional coupling between glial cells and between neurons.
2001,
Pubmed Robinson,
Unidirectional coupling of gap junctions between neuroglia.
1993,
Pubmed Rodriguez,
Origin of the precerebellar system.
2000,
Pubmed Sandri,
Membrane morphology of the vertebrate nervous system. A study with freeze-etch technique.
1977,
Pubmed Scherer,
Connexin32 is a myelin-related protein in the PNS and CNS.
1995,
Pubmed Scherer,
Connexin32-null mice develop demyelinating peripheral neuropathy.
1998,
Pubmed Scherer,
Differential regulation of the 2',3'-cyclic nucleotide 3'-phosphodiesterase gene during oligodendrocyte development.
1994,
Pubmed Shillito,
Acquired neuromyotonia: evidence for autoantibodies directed against K+ channels of peripheral nerves.
1995,
Pubmed Smart,
Deletion of the K(V)1.1 potassium channel causes epilepsy in mice.
1998,
Pubmed Söhl,
The mouse gap junction gene connexin29 is highly expressed in sciatic nerve and regulated during brain development.
2001,
Pubmed Srinivas,
Functional properties of channels formed by the neuronal gap junction protein connexin36.
1999,
Pubmed Sternberger,
Myelin-associated glycoprotein demonstrated immunocytochemically in myelin and myelin-forming cells of developing rat.
1979,
Pubmed Stolinski,
Distribution of particle aggregates in the internodal axolemma and adaxonal Schwann cell membrane of rodent peripheral nerve.
1985,
Pubmed Swenson,
Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.
1989,
Pubmed
,
Xenbase Tetzlaff,
Tight junction contact events and temporary gap junctions in the sciatic nerve fibres of the chicken during Wallerian degeneration and subsequent regeneration.
1982,
Pubmed Teubner,
Functional expression of the new gap junction gene connexin47 transcribed in mouse brain and spinal cord neurons.
2001,
Pubmed
,
Xenbase Trapp,
Immunocytochemical localization of the myelin-associated glycoprotein. Fact or artifact?
1984,
Pubmed Veenstra,
Multiple connexins confer distinct regulatory and conductance properties of gap junctions in developing heart.
1992,
Pubmed Venance,
Homotypic and heterotypic coupling mediated by gap junctions during glial cell differentiation in vitro.
1995,
Pubmed Verselis,
Connexin hemichannels and cell-cell channels: comparison of properties.
2000,
Pubmed
,
Xenbase Waxman,
Freeze-fracture ultrastructure of the perinodal astrocyte and associated glial junctions.
1984,
Pubmed White,
Genetic diseases and gene knockouts reveal diverse connexin functions.
1999,
Pubmed White,
Unique and redundant connexin contributions to lens development.
2002,
Pubmed Willecke,
Characterization of targeted connexin32-deficient mice: a model for the human Charcot-Marie-Tooth (X-type) inherited disease.
1999,
Pubmed Yoshimura,
Connexin43 is another gap junction protein in the peripheral nervous system.
1996,
Pubmed Zhao,
Characteristics of gap junction channels in Schwann cells from wild-type and connexin-null mice.
1999,
Pubmed
