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Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the location of barrier leaks. Therefore, we previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier restoration and actin accumulation following laser injury. ZnUMBA can also be effectively utilized in developing zebrafish embryos as well as cultured monolayers of Madin-Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful and flexible method that, with minimal optimization, can be applied to multiple systems to measure dynamic changes in barrier function with spatiotemporal precision.
Amasheh,
Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells.
2002, Pubmed
Amasheh,
Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells.
2002,
Pubmed Bement,
Activator-inhibitor coupling between Rho signalling and actin assembly makes the cell cortex an excitable medium.
2015,
Pubmed
,
Xenbase Boerner,
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model.
2021,
Pubmed Bolinger,
Occludin S471 Phosphorylation Contributes to Epithelial Monolayer Maturation.
2016,
Pubmed Cario,
Toll-like receptor 2 controls mucosal inflammation by regulating epithelial barrier function.
2007,
Pubmed Cavanaugh,
RhoA Mediates Epithelial Cell Shape Changes via Mechanosensitive Endocytosis.
2020,
Pubmed Chan,
Hydraulic control of mammalian embryo size and cell fate.
2019,
Pubmed Chumki,
p115RhoGEF activates RhoA to support tight junction maintenance and remodeling.
2022,
Pubmed Claude,
Fracture faces of zonulae occludentes from "tight" and "leaky" epithelia.
1973,
Pubmed Dubrovskyi,
Measurement of local permeability at subcellular level in cell models of agonist- and ventilator-induced lung injury.
2013,
Pubmed FARQUHAR,
Junctional complexes in various epithelia.
1963,
Pubmed Fukazawa,
poky/chuk/ikk1 is required for differentiation of the zebrafish embryonic epidermis.
2010,
Pubmed Furuse,
Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin.
1998,
Pubmed Furuse,
Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells.
2001,
Pubmed Furuta,
Hypoxia-inducible factor 1-dependent induction of intestinal trefoil factor protects barrier function during hypoxia.
2001,
Pubmed Ghim,
Visualization of three pathways for macromolecule transport across cultured endothelium and their modification by flow.
2017,
Pubmed Günzel,
Claudins and the modulation of tight junction permeability.
2013,
Pubmed Hatte,
Tight junctions negatively regulate mechanical forces applied to adherens junctions in vertebrate epithelial tissue.
2018,
Pubmed
,
Xenbase Higashi,
Molecular organization, regulation and function of tricellular junctions.
2020,
Pubmed Higashi,
Tricellular junctions: how to build junctions at the TRICkiest points of epithelial cells.
2017,
Pubmed Higashi,
Maintenance of the Epithelial Barrier and Remodeling of Cell-Cell Junctions during Cytokinesis.
2016,
Pubmed
,
Xenbase Higashi,
EpCAM proteolysis and release of complexed claudin-7 repair and maintain the tight junction barrier.
2023,
Pubmed Iioka,
Essential role of MARCKS in cortical actin dynamics during gastrulation movements.
2004,
Pubmed
,
Xenbase Ivanov,
Cytoskeletal regulation of epithelial barrier function during inflammation.
2010,
Pubmed Kimmel,
Stages of embryonic development of the zebrafish.
1995,
Pubmed
,
Xenbase Kollmar,
Expression and phylogeny of claudins in vertebrate primordia.
2001,
Pubmed Lim,
Confocal endomicroscopy identifies loss of local barrier function in the duodenum of patients with Crohn's disease and ulcerative colitis.
2014,
Pubmed Loh,
Extensive expansion of the claudin gene family in the teleost fish, Fugu rubripes.
2004,
Pubmed Luissint,
Inflammation and the Intestinal Barrier: Leukocyte-Epithelial Cell Interactions, Cell Junction Remodeling, and Mucosal Repair.
2016,
Pubmed Masuda,
LSR defines cell corners for tricellular tight junction formation in epithelial cells.
2011,
Pubmed Matter,
Functional analysis of tight junctions.
2003,
Pubmed Merzdorf,
Formation of functional tight junctions in Xenopus embryos.
1998,
Pubmed
,
Xenbase Monaco,
The Epithelial Cell Leak Pathway.
2021,
Pubmed Oda,
The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts.
2020,
Pubmed Quiros,
RhoGTPases, actomyosin signaling and regulation of the epithelial Apical Junctional Complex.
2014,
Pubmed Raleigh,
Occludin S408 phosphorylation regulates tight junction protein interactions and barrier function.
2011,
Pubmed Ran,
Genome engineering using the CRISPR-Cas9 system.
2013,
Pubmed Rasmussen,
Confocal Laser Endomicroscopy in Inflammatory Bowel Disease--A Systematic Review.
2015,
Pubmed Reyes,
Anillin regulates cell-cell junction integrity by organizing junctional accumulation of Rho-GTP and actomyosin.
2014,
Pubmed
,
Xenbase Richter,
A novel method for imaging sites of paracellular passage of macromolecules in epithelial sheets.
2016,
Pubmed Richter,
Probing the leak pathway: Live-cell imaging of macromolecule passage through epithelia.
2022,
Pubmed Saito,
Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function.
2021,
Pubmed Saitou,
Mammalian occludin in epithelial cells: its expression and subcellular distribution.
1997,
Pubmed Sasaki,
Dynamic behavior of paired claudin strands within apposing plasma membranes.
2003,
Pubmed Schumann,
Cell polarity-determining proteins Par-3 and PP-1 are involved in epithelial tight junction defects in coeliac disease.
2012,
Pubmed Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase Shao,
Zinc enhances intestinal epithelial barrier function through the PI3K/AKT/mTOR signaling pathway in Caco-2 cells.
2017,
Pubmed Shen,
Tight junction pore and leak pathways: a dynamic duo.
2011,
Pubmed Siddiqui,
The tight junction component Claudin E is required for zebrafish epiboly.
2010,
Pubmed Srinivasan,
TEER measurement techniques for in vitro barrier model systems.
2015,
Pubmed Stephenson,
Rho Flares Repair Local Tight Junction Leaks.
2019,
Pubmed
,
Xenbase Sugawara,
Angulin-1 seals tricellular contacts independently of tricellulin and claudins.
2021,
Pubmed Suzuki,
Crystal structure of a claudin provides insight into the architecture of tight junctions.
2014,
Pubmed Tamura,
Megaintestine in claudin-15-deficient mice.
2008,
Pubmed Tokuda,
Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells.
2015,
Pubmed Turner,
The role of molecular remodeling in differential regulation of tight junction permeability.
2014,
Pubmed Van Itallie,
Architecture of tight junctions and principles of molecular composition.
2014,
Pubmed Van Itallie,
The density of small tight junction pores varies among cell types and is increased by expression of claudin-2.
2008,
Pubmed Van Itallie,
Visualizing the dynamic coupling of claudin strands to the actin cytoskeleton through ZO-1.
2017,
Pubmed Varadarajan,
Multiscale dynamics of tight junction remodeling.
2019,
Pubmed Varadarajan,
Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.
2022,
Pubmed Wang,
Zinc supplementation modifies tight junctions and alters barrier function of CACO-2 human intestinal epithelial layers.
2013,
Pubmed Weber,
Dynamic modeling of the tight junction pore pathway.
2017,
Pubmed Wlizla,
Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos.
2018,
Pubmed
,
Xenbase Xiao,
Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway.
2018,
Pubmed Yamamoto,
Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis.
2021,
Pubmed
,
Xenbase Zeng,
Dual-Role Membrane as NH3 Permselective Reactor and Azeotrope Separator in Urea Alcoholysis.
2019,
Pubmed Zihni,
Tight junctions: from simple barriers to multifunctional molecular gates.
2016,
Pubmed
