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BACKGROUND: Glutamine-fructose-6-phosphate aminotransferase (GFPT) is a key factor in the hexosamine metabolism pathway. It regulates the downstream factor O-GlcNAc to change cell function and plays an important role in the metabolism and immune process of tissues and organs. However, the evolutionary relationship of GFPT family proteins in vertebrates has not been elucidated.
OBJECTIVE: To deduce and explore the evolution and function of vertebrate GFPT family.
METHODS: 18 GFPT sequences were obtained from Homo sapiens (H. sapiens), Trachypithecus francoisi (T. francoisi), Mus musculus (M. musculus), Rattus norvegicus (R. norvegicus), Gallus gallus (G. gallus), Zootoca vivipara (Z. vivipara), Xenopus tropicalis (X. tropicalis), Danio rerio (D. rerio), Rhincodon typus (R. typus), Plasmodium relictum from National Center for Biotechnology Information (NCBI). The physical and chemical characteristics and molecular evolution of GFPT family proteins and nucleic acid sequences were analyzed by ClustalX2, Gene Doc, MEGA-X, SMART, Datamonkey, R etc. RESULTS: Based on the neighbor-joining (NJ) phylogenetic tree and evolution fingerprints, GFPT family members of vertebrates can be divided into two groups: the GFPT1 group and the GFPT2 group. Seven positive selection sites were identified by IFEL and integrated methods mixed effects model of evolution (MEME) and fixed effects likelihood (REL). Finally, we predicted 28 phosphorylation sites and 18 ubiquitousness sites in the human GFPT1 sequence, 10 phosphorylation sites, and five ubiquitousness sites in GFPT2. Gene ontology (GO) analyzes the protein molecules and KEGG signaling pathways of vertebrates interacting with GFPT family proteins.
CONCLUSIONS: Our work confirmed that higher animals GFPT family may have differentiated GFPT1 and GFPT2, which meets their own functional needs. This knowledge answers the question what the origin and evolution of GFPT family in vertebrates and provided the basis for disease treatment and function research of GFPT protein.
Aris-Brosou,
A Not-So-Long Introduction to Computational Molecular Evolution.
2019, Pubmed
Aris-Brosou,
A Not-So-Long Introduction to Computational Molecular Evolution.
2019,
Pubmed Bauché,
Mutations in GFPT1-related congenital myasthenic syndromes are associated with synaptic morphological defects and underlie a tubular aggregate myopathy with synaptopathy.
2017,
Pubmed Cvijović,
The Effect of Strong Purifying Selection on Genetic Diversity.
2018,
Pubmed Delport,
Datamonkey 2010: a suite of phylogenetic analysis tools for evolutionary biology.
2010,
Pubmed DeRossi,
Mannose Phosphate Isomerase and Mannose Regulate Hepatic Stellate Cell Activation and Fibrosis in Zebrafish and Humans.
2019,
Pubmed Finsterer,
Congenital myasthenic syndromes.
2019,
Pubmed Gélinas,
AMPK activation counteracts cardiac hypertrophy by reducing O-GlcNAcylation.
2018,
Pubmed Harrison,
Natural selection contributed to immunological differences between hunter-gatherers and agriculturalists.
2019,
Pubmed Helman,
Leukoencephalopathy due to variants in GFPT1-associated congenital myasthenic syndrome.
2019,
Pubmed Holland,
Origin and early evolution of the vertebrates: new insights from advances in molecular biology, anatomy, and palaeontology.
2001,
Pubmed Irie,
Comparative transcriptome analysis reveals vertebrate phylotypic period during organogenesis.
2011,
Pubmed
,
Xenbase Irie,
The developmental hourglass model: a predictor of the basic body plan?
2014,
Pubmed Issop,
GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.
2018,
Pubmed Juven-Gershon,
Caudal, a key developmental regulator, is a DPE-specific transcriptional factor.
2008,
Pubmed Kaushik,
Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer.
2016,
Pubmed Kirschman,
Exogenous stress hormones alter energetic and nutrient costs of development and metamorphosis.
2017,
Pubmed Kumar,
Evolutionary diagnosis method for variants in personal exomes.
2012,
Pubmed Kumar,
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
2018,
Pubmed Lam,
The hexosamine biosynthetic pathway and cancer: Current knowledge and future therapeutic strategies.
2021,
Pubmed Larkin,
Clustal W and Clustal X version 2.0.
2007,
Pubmed Letunic,
SMART: recent updates, new developments and status in 2020.
2021,
Pubmed Li,
Defective Branched-Chain Amino Acid Catabolism Disrupts Glucose Metabolism and Sensitizes the Heart to Ischemia-Reperfusion Injury.
2017,
Pubmed Martinez-Gutierrez,
Strong Purifying Selection Is Associated with Genome Streamlining in Epipelagic Marinimicrobia.
2019,
Pubmed Moloughney,
mTORC2 Responds to Glutamine Catabolite Levels to Modulate the Hexosamine Biosynthesis Enzyme GFAT1.
2016,
Pubmed Murrell,
Detecting individual sites subject to episodic diversifying selection.
2012,
Pubmed Nie,
O-GlcNAcylation, a sweet link to the pathology of diseases.
2019,
Pubmed Pond,
Adaptation to different human populations by HIV-1 revealed by codon-based analyses.
2006,
Pubmed Pond,
Evolutionary fingerprinting of genes.
2010,
Pubmed Sayeski,
The murine glutamine:fructose-6-phosphate amidotransferase-encoding cDNA sequence.
1994,
Pubmed Semmelmann,
A Fold-Independent Interface Residue Is Crucial for Complex Formation and Allosteric Signaling in Class I Glutamine Amidotransferases.
2019,
Pubmed Shimizu,
IL-8-induced O-GlcNAc modification via GLUT3 and GFAT regulates cancer stem cell-like properties in colon and lung cancer cells.
2019,
Pubmed Shir-Shapira,
Structure-Function Analysis of the Drosophila melanogaster Caudal Transcription Factor Provides Insights into Core Promoter-preferential Activation.
2015,
Pubmed Srinivasan,
Glutamine fructose-6-phosphate amidotransferase (GFAT) gene expression and activity in patients with type 2 diabetes: inter-relationships with hyperglycaemia and oxidative stress.
2007,
Pubmed Stanislauskienė,
YqfB protein from Escherichia coli: an atypical amidohydrolase active towards N4-acylcytosine derivatives.
2020,
Pubmed Szklarczyk,
STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets.
2019,
Pubmed Tran,
Chronic activation of hexosamine biosynthesis in the heart triggers pathological cardiac remodeling.
2020,
Pubmed Whitmore,
Physical mapping of the human glutamine:fructose-6-phosphate amidotransferase gene (GFPT) to chromosome 2p13.
1995,
Pubmed York,
The origin and evolution of vertebrate neural crest cells.
2020,
Pubmed Zhang,
Common variants in glutamine:fructose-6-phosphate amidotransferase 2 (GFPT2) gene are associated with type 2 diabetes, diabetic nephropathy, and increased GFPT2 mRNA levels.
2004,
Pubmed Zhang,
Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.
2021,
Pubmed Zibrova,
GFAT1 phosphorylation by AMPK promotes VEGF-induced angiogenesis.
2017,
Pubmed
