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Structure Basis for Shaping the Nse4 Protein by the Nse1 and Nse3 Dimer within the Smc5/6 Complex.
Jo A, Li S, Shin JW, Zhao X, Cho Y.
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The Smc5/6 complex facilitates chromosome replication and DNA break repair. Within this complex, a subcomplex composed of Nse1, Nse3 and Nse4 is thought to play multiple roles through DNA binding and regulating ATP-dependent activities of the complex. However, how the Nse1-Nse3-Nse4 subcomplex carries out these multiple functions remain unclear. To address this question, we determine the crystal structure of the Xenopus laevis Nse1-Nse3-Nse4 subcomplex at 1.7 Å resolution and examine how it interacts with DNA. Our structural analyses show that the Nse1-Nse3 dimer adopts a closed conformation and forms three interfaces with a segment of Nse4, forcing it into a Z-shaped conformation. The Nse1-Nse3-Nse4 structure provides an explanation for how the lung disease immunodeficiency and chromosome breakage syndrome-causing mutations could dislodge Nse4 from Nse1-Nse3. Our DNA binding and mutational analyses reveal that the N-terminal and the middle region of Nse4 contribute to DNA interaction and cell viability. Integrating our data with previous crosslink mass spectrometry data, we propose potential roles of the Nse1-Nse3-Nse4 complex in binding DNA within the Smc5/6 complex.
Adams,
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
2010, Pubmed
Adams,
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
2010,
Pubmed Adamus,
Molecular Insights into the Architecture of the Human SMC5/6 Complex.
2020,
Pubmed Barker,
The MAGE proteins: emerging roles in cell cycle progression, apoptosis, and neurogenetic disease.
2002,
Pubmed Battye,
iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM.
2011,
Pubmed Bürmann,
An asymmetric SMC-kleisin bridge in prokaryotic condensin.
2013,
Pubmed Chomez,
An overview of the MAGE gene family with the identification of all human members of the family.
2001,
Pubmed Cuylen,
Condensin structures chromosomal DNA through topological links.
2011,
Pubmed de Vries,
The HADDOCK web server for data-driven biomolecular docking.
2010,
Pubmed Doyle,
MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
2010,
Pubmed Duan,
Structural and functional insights into the roles of the Mms21 subunit of the Smc5/6 complex.
2009,
Pubmed Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed Ganji,
Real-time imaging of DNA loop extrusion by condensin.
2018,
Pubmed Gligoris,
Closing the cohesin ring: structure and function of its Smc3-kleisin interface.
2014,
Pubmed Guerineau,
Analysis of the Nse3/MAGE-binding domain of the Nse4/EID family proteins.
2012,
Pubmed Gutierrez-Escribano,
Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction.
2020,
Pubmed Haering,
Structure and stability of cohesin's Smc1-kleisin interaction.
2004,
Pubmed Haering,
Molecular architecture of SMC proteins and the yeast cohesin complex.
2002,
Pubmed Higashi,
A Structure-Based Mechanism for DNA Entry into the Cohesin Ring.
2020,
Pubmed Hudson,
Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families.
2011,
Pubmed Jones,
Protein secondary structure prediction based on position-specific scoring matrices.
1999,
Pubmed Kamada,
Molecular basis of SMC ATPase activation: role of internal structural changes of the regulatory subcomplex ScpAB.
2013,
Pubmed Kamada,
Overall Shapes of the SMC-ScpAB Complex Are Determined by Balance between Constraint and Relaxation of Its Structural Parts.
2017,
Pubmed Kanno,
The Smc5/6 Complex Is an ATP-Dependent Intermolecular DNA Linker.
2015,
Pubmed Kelley,
The Phyre2 web portal for protein modeling, prediction and analysis.
2015,
Pubmed Kschonsak,
Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.
2017,
Pubmed Lee,
Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism.
2020,
Pubmed Li,
Structural basis for Scc3-dependent cohesin recruitment to chromatin.
2018,
Pubmed Melby,
The symmetrical structure of structural maintenance of chromosomes (SMC) and MukB proteins: long, antiparallel coiled coils, folded at a flexible hinge.
1998,
Pubmed Michaelis,
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.
1997,
Pubmed Murayama,
DNA Entry into and Exit out of the Cohesin Ring by an Interlocking Gate Mechanism.
2015,
Pubmed Newman,
Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding.
2016,
Pubmed Oravcová,
Brc1 Promotes the Focal Accumulation and SUMO Ligase Activity of Smc5-Smc6 during Replication Stress.
2019,
Pubmed Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed Palecek,
Kite Proteins: a Superfamily of SMC/Kleisin Partners Conserved Across Bacteria, Archaea, and Eukaryotes.
2015,
Pubmed Palecek,
The Smc5-Smc6 DNA repair complex. bridging of the Smc5-Smc6 heads by the KLEISIN, Nse4, and non-Kleisin subunits.
2006,
Pubmed Palecek,
SMC5/6: Multifunctional Player in Replication.
2018,
Pubmed Pebernard,
Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability.
2008,
Pubmed Pei,
PROMALS3D: a tool for multiple protein sequence and structure alignments.
2008,
Pubmed Räschle,
DNA repair. Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links.
2015,
Pubmed
,
Xenbase Schneider,
NIH Image to ImageJ: 25 years of image analysis.
2012,
Pubmed Sergeant,
Composition and architecture of the Schizosaccharomyces pombe Rad18 (Smc5-6) complex.
2005,
Pubmed Serrano,
The Smc5/6 Core Complex Is a Structure-Specific DNA Binding and Compacting Machine.
2020,
Pubmed Shi,
Cryo-EM structure of the human cohesin-NIPBL-DNA complex.
2020,
Pubmed Strunnikov,
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
1995,
Pubmed Uhlmann,
SMC complexes: from DNA to chromosomes.
2016,
Pubmed van der Crabben,
Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease.
2016,
Pubmed Vondrova,
A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6.
2020,
Pubmed Wan,
Molecular Basis for Control of Diverse Genome Stability Factors by the Multi-BRCT Scaffold Rtt107.
2019,
Pubmed Wang,
Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus.
2017,
Pubmed Wells,
Evolution of condensin and cohesin complexes driven by replacement of Kite by Hawk proteins.
2017,
Pubmed Wilhelm,
SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis.
2015,
Pubmed Woo,
Structural studies of a bacterial condensin complex reveal ATP-dependent disruption of intersubunit interactions.
2009,
Pubmed Yu,
Integrative analysis reveals unique structural and functional features of the Smc5/6 complex.
2021,
Pubmed Zabrady,
Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA.
2016,
Pubmed Zhao,
A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization.
2005,
Pubmed Zheng,
Web 3DNA--a web server for the analysis, reconstruction, and visualization of three-dimensional nucleic-acid structures.
2009,
Pubmed
