Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The cytoskeleton is a dynamic, fundamental network that not only provides mechanical strength to maintain a cell's shape but also controls critical events like cell division, polarity, and movement. Thus, how the cytoskeleton is organized and dynamically regulated is critical to our understanding of countless processes. Live imaging of fluorophore-tagged cytoskeletal proteins allows us to monitor the dynamic nature of cytoskeleton components in embryonic cells. Here, we describe a protocol to monitor and analyze cytoskeletal dynamics in primary embryonic neuronal growth cones and neural crest cells obtained from Xenopus laevis embryos.
Applegate,
plusTipTracker: Quantitative image analysis software for the measurement of microtubule dynamics.
2011, Pubmed
Applegate,
plusTipTracker: Quantitative image analysis software for the measurement of microtubule dynamics.
2011,
Pubmed Belin,
Comparative analysis of tools for live cell imaging of actin network architecture.
2014,
Pubmed
,
Xenbase Berginski,
The Focal Adhesion Analysis Server: a web tool for analyzing focal adhesion dynamics.
2013,
Pubmed Burkel,
Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin.
2007,
Pubmed
,
Xenbase Danuser,
Quantitative fluorescent speckle microscopy of cytoskeleton dynamics.
2006,
Pubmed Flores,
Author Correction: Lifeact-TagGFP2 alters F-actin organization, cellular morphology and biophysical behaviour.
2019,
Pubmed Honnappa,
An EB1-binding motif acts as a microtubule tip localization signal.
2009,
Pubmed Jacquemet,
FiloQuant reveals increased filopodia density during breast cancer progression.
2017,
Pubmed Kerstein,
Mechanochemical regulation of growth cone motility.
2015,
Pubmed Legerstee,
Dynamics and distribution of paxillin, vinculin, zyxin and VASP depend on focal adhesion location and orientation.
2019,
Pubmed Lowery,
Neural Explant Cultures from Xenopus laevis.
2012,
Pubmed
,
Xenbase Mendoza,
Quantitative fluorescent speckle microscopy (QFSM) to measure actin dynamics.
2012,
Pubmed Milet,
Dissection of Xenopus laevis neural crest for in vitro explant culture or in vivo transplantation.
2014,
Pubmed
,
Xenbase Myers,
Focal adhesion kinase promotes integrin adhesion dynamics necessary for chemotropic turning of nerve growth cones.
2011,
Pubmed
,
Xenbase Riedl,
Lifeact: a versatile marker to visualize F-actin.
2008,
Pubmed Robles,
Focal adhesion kinase signaling at sites of integrin-mediated adhesion controls axon pathfinding.
2006,
Pubmed
,
Xenbase Santiago-Medina,
PAK-PIX interactions regulate adhesion dynamics and membrane protrusion to control neurite outgrowth.
2013,
Pubmed
,
Xenbase Schell,
Inositol 1,4,5-trisphosphate 3-kinase A associates with F-actin and dendritic spines via its N terminus.
2001,
Pubmed Schindelin,
Fiji: an open-source platform for biological-image analysis.
2012,
Pubmed Stepanova,
Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein).
2003,
Pubmed Stout,
Using plusTipTracker software to measure microtubule dynamics in Xenopus laevis growth cones.
2014,
Pubmed
,
Xenbase Stutchbury,
Distinct focal adhesion protein modules control different aspects of mechanotransduction.
2017,
Pubmed Tinevez,
TrackMate: An open and extensible platform for single-particle tracking.
2017,
Pubmed Worth,
Advances in imaging cell-matrix adhesions.
2010,
Pubmed
