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Neurochem Res
2017 Sep 01;429:2443-2455. doi: 10.1007/s11064-017-2242-8.
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Inwardly Rectifying K+ Currents in Cultured Oligodendrocytes from Rat Optic Nerve are Insensitive to pH.
Pérez-Samartín A, Garay E, Moctezuma JPH, Cisneros-Mejorado A, Sánchez-Gómez MV, Martel-Gallegos G, Robles-Martínez L, Canedo-Antelo M, Matute C, Arellano RO.
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Inwardly rectifying K+ (Kir) channel expression signals at an advanced stage of maturation during oligodendroglial differentiation. Knocking down their expression halts the generation of myelin and produces severe abnormalities in the central nervous system. Kir4.1 is the main subunit involved in the tetrameric structure of Kir channels in glial cells; however, the precise composition of Kir channels expressed in oligodendrocytes (OLs) remains partially unknown, as participation of other subunits has been proposed. Kir channels are sensitive to H+; thus, intracellular acidification produces Kir current inhibition. Since Kir subunits have differential sensitivity to H+, we studied the effect of intracellular acidification on Kir currents expressed in cultured OLs derived from optic nerves of 12-day-old rats. Unexpectedly, Kir currents in OLs (2-4 DIV) did not change within the pH range of 8.0-5.0, as observed when using standard whole-cell voltage-clamp recording or when preserving cytoplasmic components with the perforated patch-clamp technique. In contrast, low pH inhibited astrocyte Kir currents, which was consistent with the involvement of the Kir4.1 subunit. The H+-insensitivity expressed in OL Kir channels was not intrinsic because Kir cloning showed no difference in the sequence reported for the Kir4.1, Kir2.1, or Kir5.1 subunits. Moreover, when Kir channels were heterologously expressed in Xenopus oocytes they behaved as expected in their general properties and sensitivity to H+. It is therefore concluded that Kir channel H+-sensitivity in OLs is modulated through an extrinsic mechanism, probably by association with a modulatory component or by posttranslational modifications.
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252121 Consejo Nacional de Ciencia y Tecnología, IN205615 Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica DGAPA-UNAM, SAF2016-75292-R Ministerio de Economía y Competitividad/Fondos Europeos de Desarrollo Regional, SAF2013-45084-R Ministerio de Economía y Competitividad/Fondos Europeos de Desarrollo Regional, PRY-15-404 Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas
Arellano,
Axon-to-Glia Interaction Regulates GABAA Receptor Expression in Oligodendrocytes.
2016,
Pubmed Attali,
Characterization of delayed rectifier Kv channels in oligodendrocytes and progenitor cells.
1997,
Pubmed Barres,
Ion channel expression by white matter glia: the O-2A glial progenitor cell.
1990,
Pubmed Barres,
Cell death and control of cell survival in the oligodendrocyte lineage.
1992,
Pubmed Berger,
Developmental changes in the membrane current pattern, K+ buffer capacity, and morphology of glial cells in the corpus callosum slice.
1991,
Pubmed Bolton,
Cyclic AMP-mediated regulation of the resting membrane potential in myelin-forming oligodendrocytes in the isolated intact rat optic nerve.
2006,
Pubmed Brasko,
Expression of Kir4.1 and Kir5.1 inwardly rectifying potassium channels in oligodendrocytes, the myelinating cells of the CNS.
2017,
Pubmed Brown,
Astrocyte glycogen as an emergency fuel under conditions of glucose deprivation or intense neural activity.
2015,
Pubmed Butt,
Inwardly rectifying potassium channels (Kir) in central nervous system glia: a special role for Kir4.1 in glial functions.
2006,
Pubmed Cahoy,
A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function.
2008,
Pubmed Cheli,
Voltage-gated Ca2+ entry promotes oligodendrocyte progenitor cell maturation and myelination in vitro.
2015,
Pubmed Chen,
Alpha 1E subunit of the R-type calcium channel is associated with myelinogenesis.
2000,
Pubmed Choe,
A conserved cytoplasmic region of ROMK modulates pH sensitivity, conductance, and gating.
1997,
Pubmed
,
Xenbase Connors,
Activity-dependent K+ accumulation in the developing rat optic nerve.
1982,
Pubmed Du,
Characteristic interactions with phosphatidylinositol 4,5-bisphosphate determine regulation of kir channels by diverse modulators.
2004,
Pubmed
,
Xenbase Fakler,
Identification of a titratable lysine residue that determines sensitivity of kidney potassium channels (ROMK) to intracellular pH.
1996,
Pubmed
,
Xenbase Hibino,
Inwardly rectifying potassium channels: their structure, function, and physiological roles.
2010,
Pubmed Hibino,
Differential assembly of inwardly rectifying K+ channel subunits, Kir4.1 and Kir5.1, in brain astrocytes.
2004,
Pubmed Hoppe,
Characteristics of activity-dependent potassium accumulation in mammalian peripheral nerve in vitro.
1991,
Pubmed Jeziorski,
Cloning and functional expression of a voltage-gated calcium channel alpha1 subunit from jellyfish.
1998,
Pubmed
,
Xenbase Kalsi,
Kir4.1 expression by astrocytes and oligodendrocytes in CNS white matter: a developmental study in the rat optic nerve.
2004,
Pubmed Kettenmann,
Intracellular pH regulation in cultured mouse oligodendrocytes.
1988,
Pubmed Kraus,
Potassium channel KIR4.1-specific antibodies in children with acquired demyelinating CNS disease.
2014,
Pubmed Kucheryavykh,
Downregulation of Kir4.1 inward rectifying potassium channel subunits by RNAi impairs potassium transfer and glutamate uptake by cultured cortical astrocytes.
2007,
Pubmed Lagrutta,
Inward rectifier potassium channels. Cloning, expression and structure-function studies.
1996,
Pubmed
,
Xenbase Larson,
Electrophysiological properties of NG2(+) cells: Matching physiological studies with gene expression profiles.
2016,
Pubmed Leng,
Subunit-subunit interactions are critical for proton sensitivity of ROMK: evidence in support of an intermolecular gating mechanism.
2006,
Pubmed
,
Xenbase Leung,
Phosphatidylinositol 4,5-bisphosphate and intracellular pH regulate the ROMK1 potassium channel via separate but interrelated mechanisms.
2000,
Pubmed
,
Xenbase Maldonado,
Oligodendrocyte precursor cells are accurate sensors of local K+ in mature gray matter.
2013,
Pubmed Marques,
Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system.
2016,
Pubmed Matute,
mRNA coding for neurotransmitter receptors in a human astrocytoma.
1992,
Pubmed
,
Xenbase Morihata,
Early and late activation of the voltage-gated proton channel during lactic acidosis through pH-dependent and -independent mechanisms.
2008,
Pubmed Moroni,
Developmental expression of Kir4.1 in astrocytes and oligodendrocytes of rat somatosensory cortex and hippocampus.
2015,
Pubmed Neusch,
Kir4.1 potassium channel subunit is crucial for oligodendrocyte development and in vivo myelination.
2001,
Pubmed Neusch,
Lack of the Kir4.1 channel subunit abolishes K+ buffering properties of astrocytes in the ventral respiratory group: impact on extracellular K+ regulation.
2006,
Pubmed Olsen,
Functional implications for Kir4.1 channels in glial biology: from K+ buffering to cell differentiation.
2008,
Pubmed Olsen,
Functional expression of Kir4.1 channels in spinal cord astrocytes.
2006,
Pubmed Orkand,
Extracellular potassium accumulation in the nervous system.
1980,
Pubmed Paynter,
Random mutagenesis screening indicates the absence of a separate H(+)-sensor in the pH-sensitive Kir channels.
2010,
Pubmed Poopalasundaram,
Glial heterogeneity in expression of the inwardly rectifying K(+) channel, Kir4.1, in adult rat CNS.
2000,
Pubmed Ransom,
Biophysical and pharmacological characterization of inwardly rectifying K+ currents in rat spinal cord astrocytes.
1995,
Pubmed Ransom,
Glial modulation of neural excitability mediated by extracellular pH: a hypothesis.
1992,
Pubmed Rapedius,
Control of pH and PIP2 gating in heteromeric Kir4.1/Kir5.1 channels by H-Bonding at the helix-bundle crossing.
2007,
Pubmed Rapedius,
Structural and functional analysis of the putative pH sensor in the Kir1.1 (ROMK) potassium channel.
2006,
Pubmed
,
Xenbase Schulte,
pH gating of ROMK (K(ir)1.1) channels: control by an Arg-Lys-Arg triad disrupted in antenatal Bartter syndrome.
1999,
Pubmed Sepúlveda,
Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels.
2015,
Pubmed Søe,
Modulation of Kir4.1 and Kir4.1-Kir5.1 channels by extracellular cations.
2009,
Pubmed
,
Xenbase Sontheimer,
Heterogeneity of potassium currents in cultured oligodendrocytes.
1988,
Pubmed Sontheimer,
Channel expression correlates with differentiation stage during the development of oligodendrocytes from their precursor cells in culture.
1989,
Pubmed Sontheimer,
Voltage-dependent ion channels in glial cells.
1994,
Pubmed Soria,
Cystine/glutamate antiporter blockage induces myelin degeneration.
2016,
Pubmed Srivastava,
Potassium channel KIR4.1 as an immune target in multiple sclerosis.
2012,
Pubmed Tanemoto,
In vivo formation of a proton-sensitive K+ channel by heteromeric subunit assembly of Kir5.1 with Kir4.1.
2000,
Pubmed Tsai,
Intracellular H+ inhibits a cloned rat kidney outer medulla K+ channel expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase Wang,
Subunit stoichiometry of the Kir1.1 channel in proton-dependent gating.
2005,
Pubmed
,
Xenbase Xu,
Molecular determinants for the distinct pH sensitivity of Kir1.1 and Kir4.1 channels.
2000,
Pubmed
,
Xenbase Yang,
Biophysical and molecular mechanisms underlying the modulation of heteromeric Kir4.1-Kir5.1 channels by CO2 and pH.
2000,
Pubmed
,
Xenbase Yuan,
Regulation of inwardly rectifying K+ channels in retinal pigment epithelial cells by intracellular pH.
2003,
Pubmed
