Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO Rep
2013 Dec 01;1412:1098-103. doi: 10.1038/embor.2013.158.
Show Gene links
Show Anatomy links
Nucleosome sliding by Chd1 does not require rigid coupling between DNA-binding and ATPase domains.
Nodelman IM, Bowman GD.
???displayArticle.abstract???
Chromatin remodellers are ATP-dependent motor proteins that physically reposition and reorganize nucleosomes. Chd1 and Iswi-type remodellers possess a DNA-binding domain (DBD) needed for efficient nucleosome mobilization; however, it has not been clear how this domain physically contributes to remodelling. Here we show that the Chd1 DBD promotes nucleosome sliding simply by tethering the remodeller to nucleosome substrates. Nucleosome sliding activity was largely resistant to increasing length and flexibility of the linker connecting the DBD and ATPase motor, arguing that the ATPase motor does not shift DNA onto the nucleosome by pulling on the DBD.
Bertagna,
The effects of conformational heterogeneity on the binding of the Notch intracellular domain to effector proteins: a case of biologically tuned disorder.
2008, Pubmed
Bertagna,
The effects of conformational heterogeneity on the binding of the Notch intracellular domain to effector proteins: a case of biologically tuned disorder.
2008,
Pubmed Cairns,
Chromatin remodeling: insights and intrigue from single-molecule studies.
2007,
Pubmed Cairns,
The logic of chromatin architecture and remodelling at promoters.
2009,
Pubmed Clapier,
Regulation of ISWI involves inhibitory modules antagonized by nucleosomal epitopes.
2012,
Pubmed Deindl,
ISWI remodelers slide nucleosomes with coordinated multi-base-pair entry steps and single-base-pair exit steps.
2013,
Pubmed Forné,
Probing the conformation of the ISWI ATPase domain with genetically encoded photoreactive crosslinkers and mass spectrometry.
2012,
Pubmed Grüne,
Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI.
2003,
Pubmed Hauk,
The chromodomains of the Chd1 chromatin remodeler regulate DNA access to the ATPase motor.
2010,
Pubmed Hota,
Diversity of operation in ATP-dependent chromatin remodelers.
2011,
Pubmed Hota,
Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains.
2013,
Pubmed Kiianitsa,
NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities.
2003,
Pubmed Li,
The role of chromatin during transcription.
2007,
Pubmed Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed Ludwigsen,
No need for a power stroke in ISWI-mediated nucleosome sliding.
2013,
Pubmed Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase McKnight,
Extranucleosomal DNA binding directs nucleosome sliding by Chd1.
2011,
Pubmed Mueller-Planitz,
The ATPase domain of ISWI is an autonomous nucleosome remodeling machine.
2013,
Pubmed Patel,
Identification of residues in chromodomain helicase DNA-binding protein 1 (Chd1) required for coupling ATP hydrolysis to nucleosome sliding.
2011,
Pubmed Patel,
Decoupling nucleosome recognition from DNA binding dramatically alters the properties of the Chd1 chromatin remodeler.
2013,
Pubmed Ryan,
Snf2-family proteins: chromatin remodellers for any occasion.
2011,
Pubmed Ryan,
The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains.
2011,
Pubmed Saha,
Chromatin remodelling: the industrial revolution of DNA around histones.
2006,
Pubmed Stockdale,
Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes.
2006,
Pubmed
,
Xenbase Yang,
The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing.
2006,
Pubmed Zweifel,
Structure and stability of the ankyrin domain of the Drosophila Notch receptor.
2003,
Pubmed
