Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Curr Biol
2015 Jan 05;251:69-74. doi: 10.1016/j.cub.2014.10.055.
Show Gene links
Show Anatomy links
A spinal opsin controls early neural activity and drives a behavioral light response.
Friedmann D, Hoagland A, Berlin S, Isacoff EY.
???displayArticle.abstract???
Nonvisual detection of light by the vertebrate hypothalamus, pineal, and retina is known to govern seasonal and circadian behaviors. However, the expression of opsins in multiple other brain structures suggests a more expansive repertoire for light regulation of physiology, behavior, and development. Translucent zebrafish embryos express extraretinal opsins early on, at a time when spontaneous activity in the developing CNS plays a role in neuronal maturation and circuit formation. Though the presence of extraretinal opsins is well documented, the function of direct photoreception by the CNS remains largely unknown. Here, we show that early activity in the zebrafish spinal central pattern generator (CPG) and the earliest locomotory behavior are dramatically inhibited by physiological levels of environmental light. We find that the photosensitivity of this circuit is conferred by vertebrate ancient long opsin A (VALopA), which we show to be a Gα(i)-coupled receptor that is expressed in the neurons of the spinal network. Sustained photoactivation of VALopA not only suppresses spontaneous activity but also alters the maturation of time-locked correlated network patterns. These results uncover a novel role for nonvisual opsins and a mechanism for environmental regulation of spontaneous motor behavior and neural activity in a circuit previously thought to be governed only by intrinsic developmental programs.
Ackman,
Retinal waves coordinate patterned activity throughout the developing visual system.
2012, Pubmed
Ackman,
Retinal waves coordinate patterned activity throughout the developing visual system.
2012,
Pubmed Akerboom,
Optimization of a GCaMP calcium indicator for neural activity imaging.
2012,
Pubmed Ben-Ari,
Giant synaptic potentials in immature rat CA3 hippocampal neurones.
1989,
Pubmed Cheng,
Intrinsic light response of retinal horizontal cells of teleosts.
2009,
Pubmed Chhetri,
Zebrafish--on the move towards ophthalmological research.
2014,
Pubmed Crisp,
Endogenous patterns of activity are required for the maturation of a motor network.
2011,
Pubmed Engeszer,
Zebrafish in the wild: a review of natural history and new notes from the field.
2007,
Pubmed Fernandes,
Enlightening the brain: linking deep brain photoreception with behavior and physiology.
2013,
Pubmed Fernandes,
Deep brain photoreceptors control light-seeking behavior in zebrafish larvae.
2012,
Pubmed Ferreira,
Silencing of odorant receptor genes by G protein βγ signaling ensures the expression of one odorant receptor per olfactory sensory neuron.
2014,
Pubmed Garaschuk,
Large-scale oscillatory calcium waves in the immature cortex.
2000,
Pubmed Gu,
Spontaneous neuronal calcium spikes and waves during early differentiation.
1994,
Pubmed
,
Xenbase Guemez-Gamboa,
Non-cell-autonomous mechanism of activity-dependent neurotransmitter switching.
2014,
Pubmed
,
Xenbase Inada,
Targeted knockdown of an opsin gene inhibits the swimming behaviour photoresponse of ascidian larvae.
2003,
Pubmed Kastanenka,
In vivo activation of channelrhodopsin-2 reveals that normal patterns of spontaneous activity are required for motoneuron guidance and maintenance of guidance molecules.
2010,
Pubmed Kirkby,
A role for correlated spontaneous activity in the assembly of neural circuits.
2013,
Pubmed Kleindienst,
Activity-dependent clustering of functional synaptic inputs on developing hippocampal dendrites.
2011,
Pubmed Kojima,
Differential expression of duplicated VAL-opsin genes in the developing zebrafish.
2008,
Pubmed Kokel,
Identification of nonvisual photomotor response cells in the vertebrate hindbrain.
2013,
Pubmed Kusakabe,
Ci-opsin1, a vertebrate-type opsin gene, expressed in the larval ocellus of the ascidian Ciona intestinalis.
2001,
Pubmed Li,
Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin.
2005,
Pubmed Lüscher,
Emerging roles for G protein-gated inwardly rectifying potassium (GIRK) channels in health and disease.
2010,
Pubmed Nissilä,
Encephalopsin (OPN3) protein abundance in the adult mouse brain.
2012,
Pubmed Peirson,
The evolution of irradiance detection: melanopsin and the non-visual opsins.
2009,
Pubmed Penn,
Competition in retinogeniculate patterning driven by spontaneous activity.
1998,
Pubmed Pietri,
Glutamate drives the touch response through a rostral loop in the spinal cord of zebrafish embryos.
2009,
Pubmed Pineda,
Knockdown of Nav1.6a Na+ channels affects zebrafish motoneuron development.
2006,
Pubmed Plazas,
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3.
2013,
Pubmed Reuveny,
Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits.
1994,
Pubmed
,
Xenbase Saint-Amant,
Synchronization of an embryonic network of identified spinal interneurons solely by electrical coupling.
2001,
Pubmed Saint-Amant,
Development of motor rhythms in zebrafish embryos.
2010,
Pubmed Sato,
Vertebrate ancient-long opsin has molecular properties intermediate between those of vertebrate and invertebrate visual pigments.
2011,
Pubmed
,
Xenbase Scott,
Targeting neural circuitry in zebrafish using GAL4 enhancer trapping.
2007,
Pubmed Tong,
Pacemaker and plateau potentials shape output of a developing locomotor network.
2012,
Pubmed Warp,
Emergence of patterned activity in the developing zebrafish spinal cord.
2012,
Pubmed West,
Pertussis toxin-catalyzed ADP-ribosylation of transducin. Cysteine 347 is the ADP-ribose acceptor site.
1985,
Pubmed Wyart,
Optogenetic dissection of a behavioural module in the vertebrate spinal cord.
2009,
Pubmed Xiang,
Light-avoidance-mediating photoreceptors tile the Drosophila larval body wall.
2010,
Pubmed Yu,
Preferential electrical coupling regulates neocortical lineage-dependent microcircuit assembly.
2012,
Pubmed
