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Biophys J
2013 Mar 05;1045:1081-8. doi: 10.1016/j.bpj.2013.01.019.
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Clipping of flexible tails of histones H3 and H4 affects the structure and dynamics of the nucleosome.
Nurse NP, Jimenez-Useche I, Smith IT, Yuan C.
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Förster resonance energy transfer was used to monitor the dynamic conformations of mononucleosomes under different chromatin folding conditions to elucidate the role of the flexible N-terminal regions of H3 and H4 histones. The H3 tail was shown to partake in intranucleosomal interactions by restricting the DNA breathing motion and compacting the nucleosome. The H3 tail effects were mostly independent of the ionic strength and valency of the ions. The H4 tail was shown to not greatly affect the nucleosome conformation, but did slightly influence the relative population of the preferred conformation. The role of the H4 tail varied depending on the valency and ionic strength, suggesting that electrostatic forces play a primary role in H4 tail interactions. Interestingly, despite the H4 tail's lack of influence, when H3 and H4 tails were simultaneously clipped, a more dramatic effect was seen than when only H3 or H4 tails were clipped. The combinatorial effect of H3 and H4 tail truncation suggests a potential mechanism by which various combinations of histone tail modifications can be used to control accessibility of DNA-binding proteins to nucleosomal DNA.
Bertin,
H3 and H4 histone tails play a central role in the interactions of recombinant NCPs.
2007, Pubmed
Bertin,
H3 and H4 histone tails play a central role in the interactions of recombinant NCPs.
2007,
Pubmed Böhm,
Nucleosome accessibility governed by the dimer/tetramer interface.
2011,
Pubmed Brower-Toland,
Specific contributions of histone tails and their acetylation to the mechanical stability of nucleosomes.
2005,
Pubmed Dorigo,
Nucleosome arrays reveal the two-start organization of the chromatin fiber.
2004,
Pubmed
,
Xenbase Edelman,
Fluorescence resonance energy transfer over approximately 130 basepairs in hyperstable lac repressor-DNA loops.
2003,
Pubmed Fischle,
Histone and chromatin cross-talk.
2003,
Pubmed Gansen,
Nucleosome disassembly intermediates characterized by single-molecule FRET.
2009,
Pubmed Gordon,
The core histone N-terminal tail domains function independently and additively during salt-dependent oligomerization of nucleosomal arrays.
2005,
Pubmed
,
Xenbase Grigoryev,
Chromatin organization - the 30 nm fiber.
2012,
Pubmed Haas,
Effect of the orientation of donor and acceptor on the probability of energy transfer involving electronic transitions of mixed polarization.
1978,
Pubmed Handwerger,
Cajal bodies, nucleoli, and speckles in the Xenopus oocyte nucleus have a low-density, sponge-like structure.
2005,
Pubmed
,
Xenbase Jenuwein,
Translating the histone code.
2001,
Pubmed Jimenez-Useche,
The effect of DNA CpG methylation on the dynamic conformation of a nucleosome.
2012,
Pubmed Kan,
The H4 tail domain participates in intra- and internucleosome interactions with protein and DNA during folding and oligomerization of nucleosome arrays.
2009,
Pubmed Kan,
The H3 tail domain participates in multiple interactions during folding and self-association of nucleosome arrays.
2007,
Pubmed
,
Xenbase Kato,
Characterization of the N-terminal tail domain of histone H3 in condensed nucleosome arrays by hydrogen exchange and NMR.
2009,
Pubmed Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase Li,
Nucleosomes facilitate their own invasion.
2004,
Pubmed
,
Xenbase Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase Miyagi,
Dynamics of nucleosomes assessed with time-lapse high-speed atomic force microscopy.
2011,
Pubmed Montezinho,
Quantification and localization of intracellular free mg in bovine chromaffin cells.
2002,
Pubmed Neumann,
Genetically encoding N(epsilon)-acetyllysine in recombinant proteins.
2008,
Pubmed Polach,
Effects of core histone tail domains on the equilibrium constants for dynamic DNA site accessibility in nucleosomes.
2000,
Pubmed Richmond,
The structure of DNA in the nucleosome core.
2003,
Pubmed Santos-Rosa,
Histone H3 tail clipping regulates gene expression.
2009,
Pubmed Stühmeier,
Global structure of three-way DNA junctions with and without additional unpaired bases: a fluorescence resonance energy transfer analysis.
1997,
Pubmed Tóth,
Chromatin compaction at the mononucleosome level.
2006,
Pubmed Voltz,
Unwrapping of nucleosomal DNA ends: a multiscale molecular dynamics study.
2012,
Pubmed Wang,
Acetylation mimics within individual core histone tail domains indicate distinct roles in regulating the stability of higher-order chromatin structure.
2008,
Pubmed
,
Xenbase Wu,
Histone H3R17me2a mark recruits human RNA polymerase-associated factor 1 complex to activate transcription.
2012,
Pubmed Wysocka,
A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling.
2006,
Pubmed
,
Xenbase Yang,
Structure and binding of the H4 histone tail and the effects of lysine 16 acetylation.
2011,
Pubmed Yang,
Biophysical analysis and small-angle X-ray scattering-derived structures of MeCP2-nucleosome complexes.
2011,
Pubmed You,
Measuring thermodynamic details of DNA hybridization using fluorescence.
2011,
Pubmed Zheng,
Salt-dependent intra- and internucleosomal interactions of the H3 tail domain in a model oligonucleosomal array.
2005,
Pubmed
,
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