Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2013 Aug 06;11032:13008-13. doi: 10.1073/pnas.1305563110.
Show Gene links
Show Anatomy links
Importance of lipid-pore loop interface for potassium channel structure and function.
van der Cruijsen EA, Nand D, Weingarth M, Prokofyev A, Hornig S, Cukkemane AA, Bonvin AM, Becker S, Hulse RE, Perozo E, Pongs O, Baldus M.
???displayArticle.abstract???
Potassium (i.e., K(+)) channels allow for the controlled and selective passage of potassium ions across the plasma membrane via a conserved pore domain. In voltage-gated K(+) channels, gating is the result of the coordinated action of two coupled gates: an activation gate at the intracellular entrance of the pore and an inactivation gate at the selectivity filter. By using solid-state NMR structural studies, in combination with electrophysiological experiments and molecular dynamics simulations, we show that the turret region connecting the outer transmembrane helix (transmembrane helix 1) and the pore helix behind the selectivity filter contributes to K(+) channel inactivation and exhibits a remarkable structural plasticity that correlates to K(+) channel inactivation. The transmembrane helix 1 unwinds when the K(+) channel enters the inactivated state and rewinds during the transition to the closed state. In addition to well-characterized changes at the K(+) ion coordination sites, this process is accompanied by conformational changes within the turret region and the pore helix. Further spectroscopic and computational results show that the same channel domain is critically involved in establishing functional contacts between pore domain and the cellular membrane. Taken together, our results suggest that the interaction between the K(+) channel turret region and the lipid bilayer exerts an important influence on the selective passage of potassium ions via the K(+) channel pore.
Ader,
Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity.
2009, Pubmed
Ader,
Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity.
2009,
Pubmed Ader,
A structural link between inactivation and block of a K+ channel.
2008,
Pubmed Ader,
Structural rearrangements of membrane proteins probed by water-edited solid-state NMR spectroscopy.
2009,
Pubmed Baukrowitz,
Modulation of K+ current by frequency and external [K+]: a tale of two inactivation mechanisms.
1995,
Pubmed Baukrowitz,
Use-dependent blockers and exit rate of the last ion from the multi-ion pore of a K+ channel.
1996,
Pubmed Bezanilla,
How membrane proteins sense voltage.
2008,
Pubmed Broomand,
Electrostatic domino effect in the Shaker K channel turret.
2007,
Pubmed
,
Xenbase Broomand,
Molecular movement of the voltage sensor in a K channel.
2003,
Pubmed
,
Xenbase Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed Catterall,
Ion channel voltage sensors: structure, function, and pathophysiology.
2010,
Pubmed Chakrapani,
On the structural basis of modal gating behavior in K(+) channels.
2011,
Pubmed Chakrapani,
A quantitative description of KcsA gating I: macroscopic currents.
2007,
Pubmed Cordero-Morales,
Molecular determinants of gating at the potassium-channel selectivity filter.
2006,
Pubmed Cuello,
Structural mechanism of C-type inactivation in K(+) channels.
2010,
Pubmed Cuello,
Structural basis for the coupling between activation and inactivation gates in K(+) channels.
2010,
Pubmed Cuello,
Design and characterization of a constitutively open KcsA.
2010,
Pubmed de Vries,
The HADDOCK web server for data-driven biomolecular docking.
2010,
Pubmed Fung,
An improved broadband decoupling sequence for liquid crystals and solids.
2000,
Pubmed Gajewski,
Biogenesis of the pore architecture of a voltage-gated potassium channel.
2011,
Pubmed Gandhi,
The orientation and molecular movement of a k(+) channel voltage-sensing domain.
2003,
Pubmed
,
Xenbase Hess,
GROMACS 4: Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation.
2008,
Pubmed Lange,
Toxin-induced conformational changes in a potassium channel revealed by solid-state NMR.
2006,
Pubmed
,
Xenbase Larsson,
A conserved glutamate is important for slow inactivation in K+ channels.
2000,
Pubmed
,
Xenbase Lee,
Two separate interfaces between the voltage sensor and pore are required for the function of voltage-dependent K(+) channels.
2009,
Pubmed
,
Xenbase Liu,
Dynamic rearrangement of the outer mouth of a K+ channel during gating.
1996,
Pubmed Long,
Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.
2007,
Pubmed Marius,
Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening.
2008,
Pubmed Nand,
Fractional deuteration applied to biomolecular solid-state NMR spectroscopy.
2012,
Pubmed Panyi,
Cross talk between activation and slow inactivation gates of Shaker potassium channels.
2006,
Pubmed Panyi,
Probing the cavity of the slow inactivated conformation of shaker potassium channels.
2007,
Pubmed Perozo,
Three-dimensional architecture and gating mechanism of a K+ channel studied by EPR spectroscopy.
1998,
Pubmed Schmidt,
Phospholipids and the origin of cationic gating charges in voltage sensors.
2006,
Pubmed Schneider,
Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers.
2008,
Pubmed Soares,
An improved nucleic acid parameter set for the GROMOS force field.
2005,
Pubmed Uysal,
Mechanism of activation gating in the full-length KcsA K+ channel.
2011,
Pubmed Uysal,
Crystal structure of full-length KcsA in its closed conformation.
2009,
Pubmed Valiyaveetil,
Lipids in the structure, folding, and function of the KcsA K+ channel.
2002,
Pubmed Wang,
Mapping the sequence of conformational changes underlying selectivity filter gating in the K(v)11.1 potassium channel.
2011,
Pubmed Weingarth,
Structural determinants of specific lipid binding to potassium channels.
2013,
Pubmed Weingarth,
Supramolecular structure of membrane-associated polypeptides by combining solid-state NMR and molecular dynamics simulations.
2012,
Pubmed Xu,
Removal of phospho-head groups of membrane lipids immobilizes voltage sensors of K+ channels.
2008,
Pubmed
,
Xenbase Yifrach,
Energetics of pore opening in a voltage-gated K(+) channel.
2002,
Pubmed
,
Xenbase Zachariae,
The molecular mechanism of toxin-induced conformational changes in a potassium channel: relation to C-type inactivation.
2008,
Pubmed
,
Xenbase Zhou,
Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution.
2001,
Pubmed
