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Nat Nanotechnol
2013 Mar 01;83:199-205. doi: 10.1038/nnano.2012.246.
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Spatiotemporal control of microtubule nucleation and assembly using magnetic nanoparticles.
Hoffmann C, Mazari E, Lallet S, Le Borgne R, Marchi V, Gosse C, Gueroui Z.
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Decisions on the fate of cells and their functions are dictated by the spatiotemporal dynamics of molecular signalling networks. However, techniques to examine the dynamics of these intracellular processes remain limited. Here, we show that magnetic nanoparticles conjugated with key regulatory proteins can artificially control, in time and space, the Ran/RCC1 signalling pathway that regulates the cell cytoskeleton. In the presence of a magnetic field, RanGTP proteins conjugated to superparamagnetic nanoparticles can induce microtubule fibres to assemble into asymmetric arrays of polarized fibres in Xenopus laevis egg extracts. The orientation of the fibres is dictated by the direction of the magnetic force. When we locally concentrated nanoparticles conjugated with the upstream guanine nucleotide exchange factor RCC1, the assembly of microtubule fibres could be induced over a greater range of distances than RanGTP particles. The method shows how bioactive nanoparticles can be used to engineer signalling networks and spatial self-organization inside a cell environment.
Athale,
Regulation of microtubule dynamics by reaction cascades around chromosomes.
2008, Pubmed,
Xenbase
Athale,
Regulation of microtubule dynamics by reaction cascades around chromosomes.
2008,
Pubmed
,
Xenbase Bashor,
Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems.
2010,
Pubmed Bastiaens,
Gradients in the self-organization of the mitotic spindle.
2006,
Pubmed
,
Xenbase Bryant,
From cells to organs: building polarized tissue.
2008,
Pubmed Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase Caudron,
Spatial coordination of spindle assembly by chromosome-mediated signaling gradients.
2005,
Pubmed
,
Xenbase Chen,
Biotechnology: remote control of living cells.
2008,
Pubmed Chiang,
Systems- and molecular-level elucidation of signaling processes through chemistry.
2008,
Pubmed Clarke,
Spatial and temporal coordination of mitosis by Ran GTPase.
2008,
Pubmed Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase Doktycz,
Nano-enabled synthetic biology.
2007,
Pubmed Ellis-Davies,
Caged compounds: photorelease technology for control of cellular chemistry and physiology.
2007,
Pubmed Gaetz,
Examining how the spatial organization of chromatin signals influences metaphase spindle assembly.
2006,
Pubmed
,
Xenbase Good,
Scaffold proteins: hubs for controlling the flow of cellular information.
2011,
Pubmed
,
Xenbase Grünberg,
Strategies for protein synthetic biology.
2010,
Pubmed Grunwald,
In situ assembly of macromolecular complexes triggered by light.
2010,
Pubmed Halpin,
Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
2011,
Pubmed
,
Xenbase Hannak,
Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts.
2006,
Pubmed
,
Xenbase Izaurralde,
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.
1997,
Pubmed Jimenez,
Towards high throughput production of artificial egg oocytes using microfluidics.
2011,
Pubmed
,
Xenbase Kalab,
The RanGTP gradient - a GPS for the mitotic spindle.
2008,
Pubmed Kaláb,
Analysis of a RanGTP-regulated gradient in mitotic somatic cells.
2006,
Pubmed
,
Xenbase Karsenti,
Self-organization in cell biology: a brief history.
2008,
Pubmed Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
,
Xenbase Kholodenko,
Signalling ballet in space and time.
2010,
Pubmed Kiel,
Engineering signal transduction pathways.
2010,
Pubmed Kinkhabwala,
Spatial aspects of intracellular information processing.
2010,
Pubmed Kumar,
Microtubule assembly: Switched on with magnets.
2013,
Pubmed Kutay,
Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.
1997,
Pubmed
,
Xenbase Lee,
Artificial control of cell signaling and growth by magnetic nanoparticles.
2010,
Pubmed Levskaya,
Spatiotemporal control of cell signalling using a light-switchable protein interaction.
2009,
Pubmed Li,
Beyond polymer polarity: how the cytoskeleton builds a polarized cell.
2008,
Pubmed Lim,
Designing customized cell signalling circuits.
2010,
Pubmed Mannix,
Nanomagnetic actuation of receptor-mediated signal transduction.
2008,
Pubmed Osterfield,
Graded positional information: interpretation for both fate and guidance.
2003,
Pubmed Pinot,
Effects of confinement on the self-organization of microtubules and motors.
2009,
Pubmed Pinot,
Confinement induces actin flow in a meiotic cytoplasm.
2012,
Pubmed
,
Xenbase Soh,
Reaction-diffusion systems in intracellular molecular transport and control.
2010,
Pubmed Steketee,
Nanoparticle-mediated signaling endosome localization regulates growth cone motility and neurite growth.
2011,
Pubmed Toettcher,
The promise of optogenetics in cell biology: interrogating molecular circuits in space and time.
2011,
Pubmed Toyoshima,
Sensitivity control through attenuation of signal transfer efficiency by negative regulation of cellular signalling.
2012,
Pubmed Vartak,
Spatial cycles in G-protein crowd control.
2010,
Pubmed Vetter,
The guanine nucleotide-binding switch in three dimensions.
2001,
Pubmed Wilner,
Enzyme cascades activated on topologically programmed DNA scaffolds.
2009,
Pubmed Wittmann,
The spindle: a dynamic assembly of microtubules and motors.
2001,
Pubmed Wu,
A genetically encoded photoactivatable Rac controls the motility of living cells.
2009,
Pubmed
