Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol
2011 Mar 15;589Pt 6:1489-503. doi: 10.1113/jphysiol.2010.200295.
Show Gene links
Show Anatomy links
Functional and developmental expression of a zebrafish Kir1.1 (ROMK) potassium channel homologue Kcnj1.
Abbas L, Hajihashemi S, Stead LF, Cooper GJ, Ware TL, Munsey TS, Whitfield TT, White SJ.
???displayArticle.abstract???
The zebrafish, Danio rerio, is emerging as an important model organism for the pathophysiological study of some human kidney diseases, but the sites of expression and physiological roles of a number of protein orthologues in the zebrafish nephron remain mostly undefined. Here we show that a zebrafish potassium channel is orthologous to the mammalian kidney potassium channel, ROMK. The cDNA (kcnj1) encodes a protein (Kcnj1) that when expressed in Xenopus laevis oocytes displayed pH- and Ba2+-sensitive K+-selective currents, but unlike the mammalian channel, was completely insensitive to the peptide inhibitor tertiapin-Q. In the pronephros, kcnj1 transcript expression was restricted to a distal region and overlapped with that of sodium–chloride cotransporter Nkcc, chloride channel ClC-Ka, and ClC-Ka/b accessory subunit Barttin, indicating the location of the diluting segment. In a subpopulation of surface cells, kcnj1 was coexpressed with the a1a.4 isoform of the Na+/K+-ATPase, identifying these cells as potential K+ secretory cells in this epithelium. At later stages of development, kcnj1 appeared in cells of the developing gill that also expressed the a1a.4 subunit.Morpholino antisense-mediated knockdown of kcnj1 was accompanied by transient tachycardia followed by bradycardia, effects consistent with alterations in extracellular K+ concentration in the embryo.Our findings indicate that Kcnj1 is expressed in cells associated with osmoregulation and acts as a K+ efflux pathway that is important in maintaining extracellular levels of K+ in the developing embryo.
Abbas,
Nkcc1 (Slc12a2) is required for the regulation of endolymph volume in the otic vesicle and swim bladder volume in the zebrafish larva.
2009, Pubmed
Abbas,
Nkcc1 (Slc12a2) is required for the regulation of endolymph volume in the otic vesicle and swim bladder volume in the zebrafish larva.
2009,
Pubmed Baker,
Defective "pacemaker" current (Ih) in a zebrafish mutant with a slow heart rate.
1997,
Pubmed Briggs,
The zebrafish: a new model organism for integrative physiology.
2002,
Pubmed Doi,
Extracellular K+ and intracellular pH allosterically regulate renal Kir1.1 channels.
1996,
Pubmed
,
Xenbase Drummond,
Reporting ethical matters in the Journal of Physiology: standards and advice.
2009,
Pubmed Estévez,
Barttin is a Cl- channel beta-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion.
2001,
Pubmed
,
Xenbase Fakler,
Identification of a titratable lysine residue that determines sensitivity of kidney potassium channels (ROMK) to intracellular pH.
1996,
Pubmed
,
Xenbase Frindt,
Dietary K regulates ROMK channels in connecting tubule and cortical collecting duct of rat kidney.
2009,
Pubmed
,
Xenbase Gamba,
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.
1994,
Pubmed
,
Xenbase Haider,
Molecular dynamics simulations of inwardly rectifying (Kir) potassium channels: a comparative study.
2007,
Pubmed Hebert,
Bartter syndrome.
2003,
Pubmed Hebert,
Molecular diversity and regulation of renal potassium channels.
2005,
Pubmed Hsiao,
A positive regulatory loop between foxi3a and foxi3b is essential for specification and differentiation of zebrafish epidermal ionocytes.
2007,
Pubmed Iwashita,
Pigment pattern in jaguar/obelix zebrafish is caused by a Kir7.1 mutation: implications for the regulation of melanosome movement.
2006,
Pubmed Jin,
A novel high-affinity inhibitor for inward-rectifier K+ channels.
1998,
Pubmed
,
Xenbase Jin,
Synthesis of a stable form of tertiapin: a high-affinity inhibitor for inward-rectifier K+ channels.
1999,
Pubmed
,
Xenbase Jin,
Mechanisms of inward-rectifier K+ channel inhibition by tertiapin-Q.
1999,
Pubmed
,
Xenbase Kahloon,
Hyperkalemia induced failure of atrial and ventricular pacemaker capture.
2005,
Pubmed Kieferle,
Two highly homologous members of the ClC chloride channel family in both rat and human kidney.
1994,
Pubmed Kimmel,
Stages of embryonic development of the zebrafish.
1995,
Pubmed
,
Xenbase Lee,
ROMK inwardly rectifying ATP-sensitive K+ channel. I. Expression in rat distal nephron segments.
1995,
Pubmed Leipziger,
PKA site mutations of ROMK2 channels shift the pH dependence to more alkaline values.
2000,
Pubmed
,
Xenbase Liao,
Expression regulation of Na+-K+-ATPase alpha1-subunit subtypes in zebrafish gill ionocytes.
2009,
Pubmed Lin,
Proton pump-rich cell secretes acid in skin of zebrafish larvae.
2006,
Pubmed Löffler,
Cation permeation and blockade of ROMK1, a cloned renal potassium channel.
1997,
Pubmed
,
Xenbase Lorenz,
Heteromultimeric CLC chloride channels with novel properties.
1996,
Pubmed
,
Xenbase Mangoni,
Genesis and regulation of the heart automaticity.
2008,
Pubmed Nishida,
Crystal structure of a Kir3.1-prokaryotic Kir channel chimera.
2007,
Pubmed Oxtoby,
Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development.
1993,
Pubmed Pan,
Epithelial Ca(2+) channel expression and Ca(2+) uptake in developing zebrafish.
2005,
Pubmed Perry,
Channels, pumps, and exchangers in the gill and kidney of freshwater fishes: their role in ionic and acid-base regulation.
2003,
Pubmed Pirovano,
PRALINETM: a strategy for improved multiple alignment of transmembrane proteins.
2008,
Pubmed Rapedius,
Structural and functional analysis of the putative pH sensor in the Kir1.1 (ROMK) potassium channel.
2006,
Pubmed
,
Xenbase Sali,
Comparative protein modelling by satisfaction of spatial restraints.
1993,
Pubmed Schulte,
K(+)-dependent gating of K(ir)1.1 channels is linked to pH gating through a conformational change in the pore.
2001,
Pubmed
,
Xenbase Schwalbe,
Potassium channel structure and function as reported by a single glycosylation sequon.
1995,
Pubmed Shin,
From Zebrafish to human: modular medical models.
2002,
Pubmed Simon,
Genetic heterogeneity of Bartter's syndrome revealed by mutations in the K+ channel, ROMK.
1996,
Pubmed Sprague,
The Zebrafish Information Network: the zebrafish model organism database.
2006,
Pubmed Varsamos,
Ontogeny of osmoregulation in postembryonic fish: a review.
2005,
Pubmed Wang,
Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio).
2009,
Pubmed Wang,
A potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop.
1990,
Pubmed Wingert,
The cdx genes and retinoic acid control the positioning and segmentation of the zebrafish pronephros.
2007,
Pubmed
,
Xenbase Xu,
Localization of the ROMK protein on apical membranes of rat kidney nephron segments.
1997,
Pubmed Zhang,
Identification and characterization of a novel member of the ATP-sensitive K+ channel subunit family, Kir6.3, in zebrafish.
2006,
Pubmed
