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In Xenopus embryos, maternal cyclins drive the first 12 cell divisions after which several cyclins are terminally degraded, including cyclin B2. Cyclin B2 disappearance is due to transcription-mediated mRNA deadenylation at the midblastula transition, when transcription initiates and the cell cycle lengthens. To further define the mechanism, we characterized proteins capable of binding cyclin B2 3'UTR. We show that ElrA and AUF1 compete for binding to regions containing cytoplasmic polyadenylation elements (CPEs), with AUF1 binding increasing at the midblastula transition. Deletion of both CPEs abrogates polyadenylation but has no effect on deadenylation or binding of ElrA or AUF1. Overexpression of ElrA or AUF1 does not alter cyclin B2 mRNA stability. These results show that ElrA and AUF1 bind to cyclin B2 mRNA independent of CPEs and function by binding other elements.
Aoki,
Xenopus cold-inducible RNA-binding protein 2 interacts with ElrA, the Xenopus homolog of HuR, and inhibits deadenylation of specific mRNAs.
2003, Pubmed,
Xenbase
Aoki,
Xenopus cold-inducible RNA-binding protein 2 interacts with ElrA, the Xenopus homolog of HuR, and inhibits deadenylation of specific mRNAs.
2003,
Pubmed
,
Xenbase Audic,
Zygotic regulation of maternal cyclin A1 and B2 mRNAs.
2001,
Pubmed
,
Xenbase Audic,
Zygotic control of maternal cyclin A1 translation and mRNA stability.
2002,
Pubmed
,
Xenbase Bhattacharyya,
Stress-induced reversal of microRNA repression and mRNA P-body localization in human cells.
2006,
Pubmed David,
FRET-detectable interactions between the ARE binding proteins, HuR and p37AUF1.
2007,
Pubmed Fan,
Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs.
1998,
Pubmed Guo,
HuR contributes to cyclin E1 deregulation in MCF-7 breast cancer cells.
2006,
Pubmed Hartley,
Screening for sequence-specific RNA-BPs by comprehensive UV crosslinking.
2002,
Pubmed
,
Xenbase Hartley,
A role for cyclin E/Cdk2 in the timing of the midblastula transition in Xenopus embryos.
1997,
Pubmed
,
Xenbase Hartley,
In vivo regulation of the early embryonic cell cycle in Xenopus.
1996,
Pubmed
,
Xenbase Howe,
A developmental timer regulates degradation of cyclin E1 at the midblastula transition during Xenopus embryogenesis.
1996,
Pubmed
,
Xenbase Howe,
Identification of a developmental timer regulating the stability of embryonic cyclin A and a new somatic A-type cyclin at gastrulation.
1995,
Pubmed
,
Xenbase Jing,
Involvement of microRNA in AU-rich element-mediated mRNA instability.
2005,
Pubmed Lal,
Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs.
2004,
Pubmed Laroia,
Control of mRNA decay by heat shock-ubiquitin-proteasome pathway.
1999,
Pubmed Lin,
Down-regulation of cyclin D1 expression by prostaglandin A(2) is mediated by enhanced cyclin D1 mRNA turnover.
2000,
Pubmed Murray,
Cell cycle extracts.
1991,
Pubmed Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase Pan,
Interaction of RNA-binding proteins HuR and AUF1 with the human ATF3 mRNA 3'-untranslated region regulates its amino acid limitation-induced stabilization.
2005,
Pubmed Sarkar,
Selective degradation of AU-rich mRNAs promoted by the p37 AUF1 protein isoform.
2003,
Pubmed Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Slevin,
ElrA binding to the 3'UTR of cyclin E1 mRNA requires polyadenylation elements.
2007,
Pubmed
,
Xenbase Sommer,
The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.
2005,
Pubmed Stebbins-Boaz,
CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Voeltz,
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase Voeltz,
AUUUA sequences direct mRNA deadenylation uncoupled from decay during Xenopus early development.
1998,
Pubmed
,
Xenbase Wang,
HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation.
2000,
Pubmed Wilson,
The search for trans-acting factors controlling messenger RNA decay.
1999,
Pubmed Wilson,
Thermodynamics and kinetics of Hsp70 association with A + U-rich mRNA-destabilizing sequences.
2001,
Pubmed Wu,
The 36-kilodalton embryonic-type cytoplasmic polyadenylation element-binding protein in Xenopus laevis is ElrA, a member of the ELAV family of RNA-binding proteins.
1997,
Pubmed
,
Xenbase
