Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Neurophysiol
2008 Oct 01;1004:2125-36. doi: 10.1152/jn.90667.2008.
Show Gene links
Show Anatomy links
Kv1 potassium channel complexes in vivo require Kvbeta2 subunits in dorsal spinal neurons.
Pineda RH, Knoeckel CS, Taylor AD, Estrada-Bernal A, Ribera AB.
???displayArticle.abstract???
Whereas Kvbeta2 subunits modulate potassium current properties carried by Kv1 channel complexes in heterologous systems, little is known about the contributions of Kvbeta2 subunits to native potassium channel function. Using antisense approaches and in situ recordings from Xenopus embryo spinal cord neurons, we tested the in vivo roles of Kvbeta2 subunits in modulation of voltage-dependent potassium current (IKv). We focused on 1) two different populations of dorsal spinal neurons that express both Kvbeta2 and Kv1 alpha-subunit genes and 2) the 24- and 48-h developmental period, during which IKv undergoes developmental regulation. At both 24 and 48 h, antisense methods produced efficient knock-down of both Kvbeta2 protein and IKv. At both times, dominant negative suppression of Kv1 channels also eliminated IKv, indicating that Kv1 channels require Kvbeta2 subunits to function in dorsal spinal neurons. Even though Kv1 channels determined the IKv values of both dorsal neuron types, comparisons of their IKv properties revealed important differences at both developmental stages. The latter results support the notion that different Kv1 alpha-subunits and/or posttranslational modifications underlie the IKv values of the two dorsal neuron types. Overall, the results demonstrate that Kvbeta2 subunits function in vivo as obligatory subunits of Kv1 channels in at least two neuron types and two different developmental stages.
Accili,
Separable Kvbeta subunit domains alter expression and gating of potassium channels.
1997, Pubmed,
Xenbase
Accili,
Separable Kvbeta subunit domains alter expression and gating of potassium channels.
1997,
Pubmed
,
Xenbase Baccaglini,
Developmental changes in the inward current of the action potential of Rohon-Beard neurones.
1977,
Pubmed
,
Xenbase Barish,
Differentiation of voltage-gated potassium current and modulation of excitability in cultured amphibian spinal neurones.
1986,
Pubmed Bekele-Arcuri,
Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides.
1996,
Pubmed Blaine,
Kv2 channels form delayed-rectifier potassium channels in situ.
2001,
Pubmed
,
Xenbase Brew,
Seizures and reduced life span in mice lacking the potassium channel subunit Kv1.2, but hypoexcitability and enlarged Kv1 currents in auditory neurons.
2007,
Pubmed Burger,
Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development.
1996,
Pubmed
,
Xenbase Campomanes,
Kv beta subunit oxidoreductase activity and Kv1 potassium channel trafficking.
2002,
Pubmed Chen,
Sh and eag K(+) channel subunit interaction in frog oocytes depends on level and time of expression.
2000,
Pubmed
,
Xenbase Chen,
Heteromultimeric interactions among K+ channel subunits from Shaker and eag families in Xenopus oocytes.
1996,
Pubmed
,
Xenbase Chouinard,
A potassium channel beta subunit related to the aldo-keto reductase superfamily is encoded by the Drosophila hyperkinetic locus.
1995,
Pubmed
,
Xenbase Coetzee,
Molecular diversity of K+ channels.
1999,
Pubmed
,
Xenbase Desarmenien,
In vivo development of voltage-dependent ionic currents in embryonic Xenopus spinal neurons.
1993,
Pubmed
,
Xenbase Desarmenien,
Role of calcium and protein kinase C in development of the delayed rectifier potassium current in Xenopus spinal neurons.
1991,
Pubmed
,
Xenbase Eisen,
Controlling morpholino experiments: don't stop making antisense.
2008,
Pubmed
,
Xenbase England,
Characterization of a voltage-gated K+ channel beta subunit expressed in human heart.
1995,
Pubmed
,
Xenbase England,
A novel K+ channel beta-subunit (hKv beta 1.3) is produced via alternative mRNA splicing.
1995,
Pubmed
,
Xenbase Gurantz,
Temporal regulation of Shaker- and Shab-like potassium channel gene expression in single embryonic spinal neurons during K+ current development.
1996,
Pubmed
,
Xenbase Gutman,
International Union of Pharmacology. XLI. Compendium of voltage-gated ion channels: potassium channels.
2003,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Heinemann,
Molecular and functional characterization of a rat brain Kv beta 3 potassium channel subunit.
1995,
Pubmed
,
Xenbase Heinemann,
The inactivation behaviour of voltage-gated K-channels may be determined by association of alpha- and beta-subunits.
1994,
Pubmed
,
Xenbase Heinemann,
Functional characterization of Kv channel beta-subunits from rat brain.
1996,
Pubmed
,
Xenbase Ikeda,
Patterned neural activity of a mutant Drosophila melanogaster.
1970,
Pubmed Jones,
Overexpression of a potassium channel gene perturbs neural differentiation.
1994,
Pubmed
,
Xenbase Kaplan,
Genetic manipulation of an abnormal jump response in Drosophila.
1974,
Pubmed Lazaroff,
Xenopus embryonic spinal neurons express potassium channel Kvbeta subunits.
1999,
Pubmed
,
Xenbase Lazaroff,
In vivo analysis of Kvbeta2 function in Xenopus embryonic myocytes.
2002,
Pubmed
,
Xenbase Li,
Voltage-gated potassium channels: regulation by accessory subunits.
2006,
Pubmed Lockery,
Reconstruction of action potential development from whole-cell currents of differentiating spinal neurons.
1992,
Pubmed Majumder,
Molecular cloning and functional expression of a novel potassium channel beta-subunit from human atrium.
1995,
Pubmed
,
Xenbase Manganas,
Subunit composition determines Kv1 potassium channel surface expression.
2000,
Pubmed McCormack,
Genetic analysis of the mammalian K+ channel beta subunit Kvbeta 2 (Kcnab2).
2002,
Pubmed
,
Xenbase Morales,
The N-terminal domain of a K+ channel beta subunit increases the rate of C-type inactivation from the cytoplasmic side of the channel.
1996,
Pubmed
,
Xenbase Nagaya,
Potassium channel alpha and beta subunits assemble in the endoplasmic reticulum.
1997,
Pubmed Nakahira,
Selective interaction of voltage-gated K+ channel beta-subunits with alpha-subunits.
1996,
Pubmed O'Dowd,
Development of voltage-dependent calcium, sodium, and potassium currents in Xenopus spinal neurons.
1988,
Pubmed
,
Xenbase Parcej,
Oligomeric properties of alpha-dendrotoxin-sensitive potassium ion channels purified from bovine brain.
1992,
Pubmed Pineda,
Dorsal-ventral gradient for neuronal plasticity in the embryonic spinal cord.
2008,
Pubmed
,
Xenbase Pineda,
Developmental, molecular, and genetic dissection of INa in vivo in embryonic zebrafish sensory neurons.
2005,
Pubmed Pongs,
Functional and molecular aspects of voltage-gated K+ channel beta subunits.
1999,
Pubmed
,
Xenbase Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase Rhodes,
Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.
1997,
Pubmed Rhodes,
Voltage-gated K+ channel beta subunits: expression and distribution of Kv beta 1 and Kv beta 2 in adult rat brain.
1996,
Pubmed Rhodes,
Association and colocalization of K+ channel alpha- and beta-subunit polypeptides in rat brain.
1995,
Pubmed Ribera,
Primary sensory neurons express a Shaker-like potassium channel gene.
1993,
Pubmed
,
Xenbase Ribera,
Zebrafish touch-insensitive mutants reveal an essential role for the developmental regulation of sodium current.
1998,
Pubmed Ribera,
A potassium channel gene is expressed at neural induction.
1990,
Pubmed
,
Xenbase Ribera,
Homogeneous development of electrical excitability via heterogeneous ion channel expression.
1996,
Pubmed
,
Xenbase Scott,
Antibodies specific for distinct Kv subunits unveil a heterooligomeric basis for subtypes of alpha-dendrotoxin-sensitive K+ channels in bovine brain.
1994,
Pubmed Shamotienko,
Subunit combinations defined for K+ channel Kv1 subtypes in synaptic membranes from bovine brain.
1997,
Pubmed Shi,
Beta subunits promote K+ channel surface expression through effects early in biosynthesis.
1996,
Pubmed Smart,
Deletion of the K(V)1.1 potassium channel causes epilepsy in mice.
1998,
Pubmed Tang,
Shaker and ether-à-go-go K+ channel subunits fail to coassemble in Xenopus oocytes.
1998,
Pubmed
,
Xenbase Torres,
A marriage of convenience: beta-subunits and voltage-dependent K+ channels.
2007,
Pubmed Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 1979.
1992,
Pubmed Trimmer,
Regulation of ion channel expression by cytoplasmic subunits.
1998,
Pubmed Wang,
In vivo functional role of the Drosophila hyperkinetic beta subunit in gating and inactivation of Shaker K+ channels.
1996,
Pubmed Wenzel,
Structural consequences of Kcna1 gene deletion and transfer in the mouse hippocampus.
2007,
Pubmed Wilson,
Interaction of the K channel beta subunit, Hyperkinetic, with eag family members.
1998,
Pubmed
,
Xenbase Xu,
Distinct functional stoichiometry of potassium channel beta subunits.
1998,
Pubmed Yang,
Kvbeta subunits increase expression of Kv4.3 channels by interacting with their C termini.
2001,
Pubmed Yao,
Auxiliary Hyperkinetic beta subunit of K+ channels: regulation of firing properties and K+ currents in Drosophila neurons.
1999,
Pubmed
