XB-ART-3732
Vaccine
2004 Apr 16;2213-14:1810-9. doi: 10.1016/j.vaccine.2003.10.052.
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Reverse transcriptase-based DNA vaccines against drug-resistant HIV-1 tested in a mouse model.
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Drug resistance is becoming a problem in the treatment of the human immunodeficiency virus type one (HIV-1). To obtain therapeutic DNA vaccines that would target multiple drug-resistance (DR) mutations, we cloned genes for DR HIV-1 reverse transcriptase (RT) and codon-optimized synthetic genes encoding clusters of human CTL epitopes located at the sites of DR-mutations (RT minigenes) and antibody and CTL-epitope tags. Expression of RT genes/minigenes in eukaryotic cells was confirmed by Western blotting and immunofluoresence staining with RT- or tag-specific antibodies. Immunization of mice with DR-RT gene induced no RT-specific antibodies. Immunization of HLA-A(*)0201-transgenic mice with RT minigenes induced RT-specific cellular responses detected by interferon-gamma secretion. This documents first steps in creating therapeutic vaccine against drug-resistant HIV strains.
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Species referenced: Xenopus laevis
Genes referenced: mhc1-uaa
GO keywords: reverse transcription
???displayArticle.disOnts??? human immunodeficiency virus infectious disease