Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
2007 Jun 01;4543:477-94. doi: 10.1007/s00424-007-0223-5.
Show Gene links
Show Anatomy links
Interaction of syntaxin with a single Kv1.1 channel: a possible mechanism for modulating neuronal excitability.
Michaelevski I, Korngreen A, Lotan I.
???displayArticle.abstract???
Voltage-gated K(+) channels are crucial for intrinsic neuronal plasticity and present a target for modulations by protein-protein interactions, notably, by exocytotic proteins demonstrated by us in several systems. Here, we investigated the interaction of a single Kv1.1 channel with syntaxin 1A. Syntaxin decreased the unitary conductance of all conductance states (two subconductances and a full conductance) and decreased their open probabilities by prolongation of mean closed dwell-times at depolarized potentials. However, at subthreshold potentials syntaxin 1A increased the probabilities of the subconductance states. Consequently, the macroscopic conductance is decreased at potentials above threshold and increased at threshold potentials. Numerical modeling based on steady-state and kinetic analyses suggests: (1) a mechanism whereby syntaxin controls activation gating by forcing the conductance pathway only via a sequence of discrete steps through the subconductance states, possibly via a breakdown of cooperative movements of voltage sensors that exist in Kv1.1; (2) a physiological effect, apparently paradoxical for an agent that reduces K(+) current, of attenuating neuronal firing frequency via an increase in K(+) shunting conductance. Such modulation of the gain of neuronal output in response to different levels of syntaxin is in accord with the suggested role for Kv1.1 in axonal excitability and synaptic efficacy.
Bajjalieh,
The biochemistry of neurotransmitter secretion.
1995, Pubmed
Bajjalieh,
The biochemistry of neurotransmitter secretion.
1995,
Pubmed Bennett,
SNAREs and the specificity of transport vesicle targeting.
1995,
Pubmed Bezanilla,
The origin of subconductance levels in voltage-gated K+ channels.
2005,
Pubmed Bezprozvanny,
Molecular determinants of the functional interaction between syntaxin and N-type Ca2+ channel gating.
2000,
Pubmed
,
Xenbase Chapman,
K channel subconductance levels result from heteromeric pore conformations.
2005,
Pubmed
,
Xenbase Chiu,
Analysis of potassium channel functions in mammalian axons by gene knockouts.
1999,
Pubmed Condliffe,
Syntaxin 1A regulates ENaC channel activity.
2004,
Pubmed
,
Xenbase Cushman,
Voltage dependent activation of potassium channels is coupled to T1 domain structure.
2000,
Pubmed Dascal,
Calcium channel currents in Xenopus oocytes injected with rat skeletal muscle RNA.
1992,
Pubmed
,
Xenbase Dodson,
Two heteromeric Kv1 potassium channels differentially regulate action potential firing.
2002,
Pubmed Fili,
Direct interaction of a brain voltage-gated K+ channel with syntaxin 1A: functional impact on channel gating.
2001,
Pubmed
,
Xenbase Geiger,
Dynamic control of presynaptic Ca(2+) inflow by fast-inactivating K(+) channels in hippocampal mossy fiber boutons.
2000,
Pubmed Grissmer,
Pharmacological characterization of five cloned voltage-gated K+ channels, types Kv1.1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines.
1994,
Pubmed Hines,
The NEURON simulation environment.
1997,
Pubmed HODGKIN,
Movement of sodium and potassium ions during nervous activity.
1952,
Pubmed HODGKIN,
Measurement of current-voltage relations in the membrane of the giant axon of Loligo.
1952,
Pubmed HODGKIN,
Propagation of electrical signals along giant nerve fibers.
1952,
Pubmed Islas,
Voltage sensitivity and gating charge in Shaker and Shab family potassium channels.
1999,
Pubmed
,
Xenbase Jarvis,
G protein modulation of N-type calcium channels is facilitated by physical interactions between syntaxin 1A and Gbetagamma.
2000,
Pubmed Jarvis,
Distinct molecular determinants govern syntaxin 1A-mediated inactivation and G-protein inhibition of N-type calcium channels.
2001,
Pubmed Keren,
Constraining compartmental models using multiple voltage recordings and genetic algorithms.
2005,
Pubmed Linial,
Deciphering neuronal secretion: tools of the trade.
1996,
Pubmed Lopantsev,
Hyperexcitability of CA3 pyramidal cells in mice lacking the potassium channel subunit Kv1.1.
2003,
Pubmed Meiri,
Reversible antisense inhibition of Shaker-like Kv1.1 potassium channel expression impairs associative memory in mouse and rat.
1997,
Pubmed Michaelevski,
Modulation of a brain voltage-gated K+ channel by syntaxin 1A requires the physical interaction of Gbetagamma with the channel.
2002,
Pubmed
,
Xenbase Qin,
Maximum likelihood estimation of aggregated Markov processes.
1997,
Pubmed Qin,
Hidden Markov modeling for single channel kinetics with filtering and correlated noise.
2000,
Pubmed Rasband,
Potassium channel distribution, clustering, and function in remyelinating rat axons.
1998,
Pubmed Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase Rhodes,
Association and colocalization of K+ channel alpha- and beta-subunit polypeptides in rat brain.
1995,
Pubmed Sheng,
Calcium-dependent interaction of N-type calcium channels with the synaptic core complex.
1996,
Pubmed Shillito,
Acquired neuromyotonia: evidence for autoantibodies directed against K+ channels of peripheral nerves.
1995,
Pubmed Singer-Lahat,
Modal behavior of the Kv1.1 channel conferred by the Kvbeta1.1 subunit and its regulation by dephosphorylation of Kv1.1.
1999,
Pubmed
,
Xenbase Stanley,
Cleavage of syntaxin prevents G-protein regulation of presynaptic calcium channels.
1997,
Pubmed Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase Südhof,
The synaptic vesicle cycle: a cascade of protein-protein interactions.
1995,
Pubmed Tan,
Modulation by K+ channels of action potential-evoked intracellular Ca2+ concentration rises in rat cerebellar basket cell axons.
1999,
Pubmed Veh,
Immunohistochemical localization of five members of the Kv1 channel subunits: contrasting subcellular locations and neuron-specific co-localizations in rat brain.
1995,
Pubmed Wang,
Heteromultimeric K+ channels in terminal and juxtaparanodal regions of neurons.
1993,
Pubmed
,
Xenbase Wang,
Hypomyelination alters K+ channel expression in mouse mutants shiverer and Trembler.
1995,
Pubmed Wang,
Voltage-dependent gating rearrangements in the intracellular T1-T1 interface of a K+ channel.
2006,
Pubmed
,
Xenbase Wang,
Localization of Kv1.1 and Kv1.2, two K channel proteins, to synaptic terminals, somata, and dendrites in the mouse brain.
1994,
Pubmed Yao,
Close association of the N terminus of Kv1.3 with the pore region.
2000,
Pubmed
,
Xenbase Yi,
Controlling potassium channel activities: Interplay between the membrane and intracellular factors.
2001,
Pubmed Zheng,
Selectivity changes during activation of mutant Shaker potassium channels.
1997,
Pubmed
,
Xenbase Zheng,
Intermediate conductances during deactivation of heteromultimeric Shaker potassium channels.
1998,
Pubmed
,
Xenbase
