Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus. A low level of gene amplification was also detected in premeiotic nuclei (oogonia).
BAUTZ,
The isolation of T4-specific RNA on a DNA-cellulose column.
1962, Pubmed
BAUTZ,
The isolation of T4-specific RNA on a DNA-cellulose column.
1962,
Pubmed BOLTON,
A general method for the isolation of RNA complementary to DNA.
1962,
Pubmed Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed CONGER,
A quick-freeze method for making smear slides permanent.
1953,
Pubmed Evans,
Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.
1968,
Pubmed
,
Xenbase Flamm,
The isolation of complementary strands from a mouse DNA fraction.
1967,
Pubmed Gall,
Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
1968,
Pubmed Gillespie,
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
1965,
Pubmed HALL,
Sequence complementarity of T2-DNA and T2-specific RNA.
1961,
Pubmed KIT,
Equilibrium sedimentation in density gradients of DNA preparations from animal tissues.
1961,
Pubmed NYGAARD,
FORMATION AND PROPERTIES OF RNA-DNA COMPLEXES.
1964,
Pubmed Sambrook,
The integrated state of viral DNA in SV40-transformed cells.
1968,
Pubmed
