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Mol Cell Biol
2007 Mar 01;276:2120-9. doi: 10.1128/MCB.01638-06.
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Structure/function analysis of Xenopus cryptochromes 1 and 2 reveals differential nuclear localization mechanisms and functional domains important for interaction with and repression of CLOCK-BMAL1.
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Circadian rhythms control the temporal arrangement of molecular, physiological, and behavioral processes within an organism and also synchronize these processes with the external environment. A cell autonomous molecular oscillator, consisting of interlocking transcriptional/translational feedback loops, drives the approximately 24-hour duration of these rhythms. The cryptochrome protein (CRY) plays a central part in the negative feedback loop of the molecular clock by translocating to the nucleus and repressing CLOCK and BMAL1, two transcription factors that comprise the positive elements in this cycle. In order to gain insight into the inner workings of this feedback loop, we investigated the structure/function relationships of Xenopus laevis CRY1 (xCRY1) and xCRY2 in cultured cells. The C-terminal tails of both xCRY1 and xCRY2 are sufficient for their nuclear localization but achieve it by different mechanisms. Through the generation and characterization of xCRY/photolyase chimeras, we found that the second half of the photolyase homology region (PHR) of CRY is important for repression through facilitating interaction with BMAL1. Characterization of these functional domains in CRYs will help us to better understand the mechanism of the known roles of CRYs and to elucidate new intricacies of the molecular clock.
Bell-Pedersen,
Circadian rhythms from multiple oscillators: lessons from diverse organisms.
2005, Pubmed
Bell-Pedersen,
Circadian rhythms from multiple oscillators: lessons from diverse organisms.
2005,
Pubmed Cashmore,
Cryptochromes: blue light receptors for plants and animals.
1999,
Pubmed Chaves,
Functional evolution of the photolyase/cryptochrome protein family: importance of the C terminus of mammalian CRY1 for circadian core oscillator performance.
2006,
Pubmed Etchegaray,
Rhythmic histone acetylation underlies transcription in the mammalian circadian clock.
2003,
Pubmed Griffin,
Light-independent role of CRY1 and CRY2 in the mammalian circadian clock.
1999,
Pubmed Guex,
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling.
1997,
Pubmed Kondratov,
BMAL1-dependent circadian oscillation of nuclear CLOCK: posttranslational events induced by dimerization of transcriptional activators of the mammalian clock system.
2003,
Pubmed Kort,
DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction.
2004,
Pubmed Kumagai,
Binding of 14-3-3 proteins and nuclear export control the intracellular localization of the mitotic inducer Cdc25.
1999,
Pubmed
,
Xenbase Kume,
mCRY1 and mCRY2 are essential components of the negative limb of the circadian clock feedback loop.
1999,
Pubmed Lee,
Posttranslational mechanisms regulate the mammalian circadian clock.
2001,
Pubmed Liu,
Circadian regulation of nocturnin transcription by phosphorylated CREB in Xenopus retinal photoreceptor cells.
2002,
Pubmed
,
Xenbase Macara,
Transport into and out of the nucleus.
2001,
Pubmed Naruse,
Circadian and light-induced transcription of clock gene Per1 depends on histone acetylation and deacetylation.
2004,
Pubmed Ripperger,
Rhythmic CLOCK-BMAL1 binding to multiple E-box motifs drives circadian Dbp transcription and chromatin transitions.
2006,
Pubmed Sakakida,
Importin alpha/beta mediates nuclear transport of a mammalian circadian clock component, mCRY2, together with mPER2, through a bipartite nuclear localization signal.
2005,
Pubmed Sato,
Feedback repression is required for mammalian circadian clock function.
2006,
Pubmed Shevchuk,
Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously.
2004,
Pubmed van der Horst,
Mammalian Cry1 and Cry2 are essential for maintenance of circadian rhythms.
1999,
Pubmed Yagita,
Nucleocytoplasmic shuttling and mCRY-dependent inhibition of ubiquitylation of the mPER2 clock protein.
2002,
Pubmed Young,
Time zones: a comparative genetics of circadian clocks.
2001,
Pubmed Zhu,
Nuclear localization and transcriptional repression are confined to separable domains in the circadian protein CRYPTOCHROME.
2003,
Pubmed
,
Xenbase Zhu,
Three cryptochromes are rhythmically expressed in Xenopus laevis retinal photoreceptors.
2001,
Pubmed
,
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