Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Han SJ, Vaccari S, Nedachi T, Andersen CB, Kovacina KS, Roth RA, Conti M.
???displayArticle.abstract???
cGMP-inhibited cAMP phosphodiesterase 3A (PDE3A) is expressed in mouse oocytes, and its function is indispensable for meiotic maturation as demonstrated by genetic ablation. Moreover, PDE3 activity is required for insulin/insulin-like growth factor-1 stimulation of Xenopus oocyte meiotic resumption. Here, we investigated the cAMP-dependent protein kinase B (PKB)/Akt regulation of PDE3A and its impact on oocyte maturation. Cell-free incubation of recombinant mouse PDE3A with PKB/Akt or cAMP-dependent protein kinase A catalytic subunits leads to phosphorylation of the PDE3A protein. Coexpression of PDE3A with constitutively activated PKB/Akt (Myr-Akt) increases PDE activity as well as its phosphorylation state. Injection of pde3a mRNA potentiates insulin-dependent maturation of Xenopus oocytes and rescues the phenotype of pde3(-/-) mouse oocytes. This effect is greatly decreased by mutation of any of the PDE3A serines 290-292 to alanine in both Xenopus and mouse. Microinjection of myr-Akt in mouse oocytes causes in vitro meiotic maturation and this effect requires PDE3A. Collectively, these data indicate that activation of PDE3A by PKB/Akt-mediated phosphorylation plays a role in the control of PDE3A activity in mammalian oocytes.
Alliston,
Expression and localization of serum/glucocorticoid-induced kinase in the rat ovary: relation to follicular growth and differentiation.
2000, Pubmed
Alliston,
Expression and localization of serum/glucocorticoid-induced kinase in the rat ovary: relation to follicular growth and differentiation.
2000,
Pubmed Andersen,
Protein kinase B/Akt induces resumption of meiosis in Xenopus oocytes.
1998,
Pubmed
,
Xenbase Andersen,
Protein kinase B/Akt is essential for the insulin- but not progesterone-stimulated resumption of meiosis in Xenopus oocytes.
2003,
Pubmed
,
Xenbase Conti,
Role of cyclic nucleotide phosphodiesterases in resumption of meiosis.
1998,
Pubmed Conti,
Role of the epidermal growth factor network in ovarian follicles.
2006,
Pubmed Conti,
Role of cyclic nucleotide signaling in oocyte maturation.
2002,
Pubmed
,
Xenbase Deuter-Reinhard,
SIP/SHIP inhibits Xenopus oocyte maturation induced by insulin and phosphatidylinositol 3-kinase.
1997,
Pubmed
,
Xenbase Downward,
PI 3-kinase, Akt and cell survival.
2004,
Pubmed El-Etr,
Meiotic maturation in Xenopus laevis oocytes initiated by insulin.
1979,
Pubmed
,
Xenbase Goud,
In-vitro maturation of human germinal vesicle stage oocytes: role of cumulus cells and epidermal growth factor in the culture medium.
1998,
Pubmed Grant,
cAMP-mediated phosphorylation of the low-Km cAMP phosphodiesterase markedly stimulates its catalytic activity.
1988,
Pubmed Han,
Wee1B is an oocyte-specific kinase involved in the control of meiotic arrest in the mouse.
2005,
Pubmed
,
Xenbase Hoshino,
Phosphatidylinositol 3-kinase and Akt participate in the FSH-induced meiotic maturation of mouse oocytes.
2004,
Pubmed Kalous,
PKB/AKT is involved in resumption of meiosis in mouse oocytes.
2006,
Pubmed Kenan,
Functions of the N-terminal region of cyclic nucleotide phosphodiesterase 3 (PDE 3) isoforms.
2000,
Pubmed Kim,
Cyclic AMP inhibits Akt activity by blocking the membrane localization of PDK1.
2001,
Pubmed Kitamura,
Insulin-induced phosphorylation and activation of cyclic nucleotide phosphodiesterase 3B by the serine-threonine kinase Akt.
1999,
Pubmed Kohn,
Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation.
1996,
Pubmed Liu,
Expression of cyclic GMP-inhibited phosphodiesterases 3A and 3B (PDE3A and PDE3B) in rat tissues: differential subcellular localization and regulated expression by cyclic AMP.
1998,
Pubmed Liu,
Molecular cloning of an amphibian insulin receptor substrate 1-like cDNA and involvement of phosphatidylinositol 3-kinase in insulin-induced Xenopus oocyte maturation.
1995,
Pubmed
,
Xenbase Macphee,
Phosphorylation results in activation of a cAMP phosphodiesterase in human platelets.
1988,
Pubmed Maller,
A study of the induction of cell division in amphibian oocytes by insulin.
1981,
Pubmed
,
Xenbase Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase Masciarelli,
Cyclic nucleotide phosphodiesterase 3A-deficient mice as a model of female infertility.
2004,
Pubmed Meijer,
Starfish oocyte maturation: evidence for a cyclic AMP-dependent inhibitory pathway.
1989,
Pubmed Movsesian,
PDE3 cyclic nucleotide phosphodiesterases and the compartmentation of cyclic nucleotide-mediated signalling in cardiac myocytes.
2002,
Pubmed Murray,
Identification of different specificity requirements between SGK1 and PKBalpha.
2005,
Pubmed Murthy,
PKA-dependent activation of PDE3A and PDE4 and inhibition of adenylyl cyclase V/VI in smooth muscle.
2002,
Pubmed Newhall,
Dynamic anchoring of PKA is essential during oocyte maturation.
2006,
Pubmed Oki,
Short term feedback regulation of cAMP in FRTL-5 thyroid cells. Role of PDE4D3 phosphodiesterase activation.
2000,
Pubmed Okumura,
Akt inhibits Myt1 in the signalling pathway that leads to meiotic G2/M-phase transition.
2002,
Pubmed Onuma,
Identification of the insulin-regulated interaction of phosphodiesterase 3B with 14-3-3 beta protein.
2002,
Pubmed Park,
Serum and glucocorticoid-inducible kinase (SGK) is a target of the PI 3-kinase-stimulated signaling pathway.
1999,
Pubmed Park,
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
2004,
Pubmed Pozuelo Rubio,
Phosphodiesterase 3A binds to 14-3-3 proteins in response to PMA-induced phosphorylation of Ser428.
2005,
Pubmed Richard,
Role of phosphodiesterase type 3A in rat oocyte maturation.
2001,
Pubmed Rondinone,
Phosphorylation of PDE3B by phosphatidylinositol 3-kinase associated with the insulin receptor.
2000,
Pubmed Sadler,
Type III phosphodiesterase plays a necessary role in the growth-promoting actions of insulin, insulin-like growth factor-I, and Ha p21ras in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase Scheid,
PKB/AKT: functional insights from genetic models.
2001,
Pubmed Sette,
Phosphorylation and activation of a cAMP-specific phosphodiesterase by the cAMP-dependent protein kinase. Involvement of serine 54 in the enzyme activation.
1996,
Pubmed Shakur,
Membrane localization of cyclic nucleotide phosphodiesterase 3 (PDE3). Two N-terminal domains are required for the efficient targeting to, and association of, PDE3 with endoplasmic reticulum.
2000,
Pubmed Shitsukawa,
Cloning and characterization of the cyclic guanosine monophosphate-inhibited phosphodiesterase PDE3A expressed in mouse oocyte.
2001,
Pubmed Smitz,
Epidermal growth factor combined with recombinant human chorionic gonadotrophin improves meiotic progression in mouse follicle-enclosed oocyte culture.
1998,
Pubmed Svoboda,
RNAi in mouse oocytes and preimplantation embryos: effectiveness of hairpin dsRNA.
2001,
Pubmed Thompson,
Assay of cyclic nucleotide phosphodiesterase and resolution of multiple molecular forms of the enzyme.
1979,
Pubmed Toker,
Cellular signaling: pivoting around PDK-1.
2000,
Pubmed Tsafriri,
Oocyte maturation involves compartmentalization and opposing changes of cAMP levels in follicular somatic and germ cells: studies using selective phosphodiesterase inhibitors.
1996,
Pubmed Webster,
Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum.
1993,
Pubmed Wechsler,
Isoforms of cyclic nucleotide phosphodiesterase PDE3A in cardiac myocytes.
2002,
Pubmed Wijkander,
Insulin-induced phosphorylation and activation of phosphodiesterase 3B in rat adipocytes: possible role for protein kinase B but not mitogen-activated protein kinase or p70 S6 kinase.
1998,
Pubmed Wiley,
Epidermal growth factor receptor mRNA and protein increase after the four-cell preimplantation stage in murine development.
1992,
Pubmed
