Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The Chinese hamster dihydrofolate reductase (DHFR) DNA replication initiation region, the 5.8 kb ori-beta, can function as a DNA replicator at random ectopic chromosomal sites in hamster cells. We report a detailed genetic analysis of the DiNucleotide Repeat (DNR) element, one of several sequence elements necessary for ectopic ori-beta activity. Deletions within ori-beta identified a 132 bp core region within the DNR element, consisting mainly of dinucleotide repeats, and a downstream region that are required for ori-beta initiation activity at non-specific ectopic sites in hamster cells. Replacement of the DNR element with Xenopus or mouse transcriptional elements from rDNA genes restored full levels of initiation activity, but replacement with a nucleosome positioning element or a viral intron sequence did not. The requirement for the DNR element and three other ori-beta sequence elements was conserved when ori-beta activity was tested at either random sites or at a single specific ectopic chromosomal site in human cells. These results confirm the importance of specific cis-acting elements in directing the initiation of DNA replication in mammalian cells, and provide new evidence that transcriptional elements can functionally substitute for one of these elements in ori-beta.
???displayArticle.pubmedLink???
17078947 ???displayArticle.pmcLink???PMC1810229 ???displayArticle.link???Exp Cell Res ???displayArticle.grants???[+]
Abdurashidova,
Localization of proteins bound to a replication origin of human DNA along the cell cycle.
2003, Pubmed
Abdurashidova,
Localization of proteins bound to a replication origin of human DNA along the cell cycle.
2003,
Pubmed Aladjem,
Genetic dissection of a mammalian replicator in the human beta-globin locus.
1998,
Pubmed Altman,
Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin.
2004,
Pubmed Altman,
The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.
2001,
Pubmed Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed Bell,
The origin recognition complex: from simple origins to complex functions.
2002,
Pubmed Berberich,
In vitro replication of plasmids containing human c-myc DNA.
1995,
Pubmed Bianchi,
Sequences near the origin of replication of the DHFR locus of Chinese hamster ovary cells adopt left-handed Z-DNA and triplex structures.
1990,
Pubmed Brinton,
Position and orientation-dependent effects of a eukaryotic Z-triplex DNA motif on episomal DNA replication in COS-7 cells.
1991,
Pubmed Caddle,
Intramolecular DNA triplexes, bent DNA and DNA unwinding elements in the initiation region of an amplified dihydrofolate reductase replicon.
1990,
Pubmed Cvetic,
Eukaryotic origins of DNA replication: could you please be more specific?
2005,
Pubmed
,
Xenbase Diffley,
Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer.
1988,
Pubmed Espinás,
Formation of triple-stranded DNA at d(GA.TC)n sequences prevents nucleosome assembly and is hindered by nucleosomes.
1996,
Pubmed Gerber,
Termination of mammalian rDNA replication: polar arrest of replication fork movement by transcription termination factor TTF-I.
1997,
Pubmed Ghosh,
Transcription factor binding and induced transcription alter chromosomal c-myc replicator activity.
2004,
Pubmed Gilbert,
In search of the holy replicator.
2004,
Pubmed Gilbert,
Making sense of eukaryotic DNA replication origins.
2001,
Pubmed Grummt,
Transcription of mouse rDNA terminates downstream of the 3' end of 28S RNA and involves interaction of factors with repeated sequences in the 3' spacer.
1985,
Pubmed Hannan,
Identification of a mammalian RNA polymerase I holoenzyme containing components of the DNA repair/replication system.
1999,
Pubmed Hyrien,
Mechanisms and consequences of replication fork arrest.
2000,
Pubmed JACOB,
[On the regulation of DNA synthesis in bacteria: the hypothesis of the replicon].
1963,
Pubmed Jin,
Non-random deletions at the dihydrofolate reductase locus of Chinese hamster ovary cells induced by alpha-particles simulating radon.
1995,
Pubmed Keller,
The origin recognition complex marks a replication origin in the human TOP1 gene promoter.
2002,
Pubmed Kennedy,
Pur-1, a zinc-finger protein that binds to purine-rich sequences, transactivates an insulin promoter in heterologous cells.
1992,
Pubmed Kobayashi,
Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone.
1998,
Pubmed Kohzaki,
Transcription factors and DNA replication origin selection.
2005,
Pubmed Ladenburger,
Identification of a binding region for human origin recognition complex proteins 1 and 2 that coincides with an origin of DNA replication.
2002,
Pubmed Längst,
RNA polymerase I transcription on nucleosomal templates: the transcription termination factor TTF-I induces chromatin remodeling and relieves transcriptional repression.
1997,
Pubmed Längst,
TTF-I determines the chromatin architecture of the active rDNA promoter.
1998,
Pubmed Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase Li,
Selective activation of pre-replication complexes in vitro at specific sites in mammalian nuclei.
2000,
Pubmed
,
Xenbase Lipford,
Nucleosomes positioned by ORC facilitate the initiation of DNA replication.
2001,
Pubmed Liu,
Multiple functional elements comprise a Mammalian chromosomal replicator.
2003,
Pubmed López-estraño,
Co-localization of polar replication fork barriers and rRNA transcription terminators in mouse rDNA.
1998,
Pubmed Machida,
Right place, right time, and only once: replication initiation in metazoans.
2005,
Pubmed Malott,
Activity of the c-myc replicator at an ectopic chromosomal location.
1999,
Pubmed Marahrens,
A yeast chromosomal origin of DNA replication defined by multiple functional elements.
1992,
Pubmed Mesner,
Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.
2005,
Pubmed Mesner,
Isolating apparently pure libraries of replication origins from complex genomes.
2006,
Pubmed Oestreich,
Regulation of TCRbeta gene assembly by a promoter/enhancer holocomplex.
2006,
Pubmed Paixão,
Modular structure of the human lamin B2 replicator.
2004,
Pubmed Putter,
Transcription termination factor TTF-I exhibits contrahelicase activity during DNA replication.
2002,
Pubmed Raghuraman,
Replication dynamics of the yeast genome.
2001,
Pubmed Rao,
Pausing in simian virus 40 DNA replication by a sequence containing (dG-dA)27.(dT-dC)27.
1988,
Pubmed Saha,
The promoter of the Chinese hamster ovary dihydrofolate reductase gene regulates the activity of the local origin and helps define its boundaries.
2004,
Pubmed Sasaki,
The Chinese hamster dihydrofolate reductase replication origin decision point follows activation of transcription and suppresses initiation of replication within transcription units.
2006,
Pubmed
,
Xenbase Sikes,
Regulation of V(D)J recombination: a dominant role for promoter positioning in gene segment accessibility.
2002,
Pubmed Song,
Structural organization and expression of the mouse gene for Pur-1, a highly conserved homolog of the human MAZ gene.
1999,
Pubmed Stillman,
DNA replication. Genomic views of genome duplication.
2001,
Pubmed Thiriet,
Functionally relevant histone-DNA interactions extend beyond the classically defined nucleosome core region.
1998,
Pubmed
,
Xenbase Todorovic,
Human origins of DNA replication selected from a library of nascent DNA.
2005,
Pubmed Wallisch,
Ku antigen supports termination of mammalian rDNA replication by transcription termination factor TTF-I.
2002,
Pubmed Wang,
The human beta-globin replication initiation region consists of two modular independent replicators.
2004,
Pubmed Wang,
Cooperative sequence modules determine replication initiation sites at the human beta-globin locus.
2006,
Pubmed Wilmes,
The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation.
2002,
Pubmed Wyrick,
Genome-wide distribution of ORC and MCM proteins in S. cerevisiae: high-resolution mapping of replication origins.
2001,
Pubmed Yan,
HBV C promoter Sp1 binding sequence functionally substitutes for the yeast ARS1 ABF1 binding site.
2002,
Pubmed Yang,
Xenopus transcription factor IIIA and the 5S nucleosome: development of a useful in vitro system.
2003,
Pubmed
,
Xenbase
