Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
1. Intra-axonal organelles were detected by darkfield and Nomarski microscopy in isolated myelinated nerve fibres from Xenopus laevis. Nerve fibres from the 8th spinal roots, the sciatic nerve, and identified motor and sensory axons from other hind limb nerves were used. The movement of the organelles was recorded either on motion picture film or by noting the times at which they crossed the lines of an ocular grid.2. Three groups of organelles were detected in all fibres. A group of particles with round profiles 0.2-0.5 mum in diameter moved somatopetally. Another group of round particles moved somatofugally. The ratio of the number of somatopetally travelling particles to the number of somatofugally travelling particles was about 10:1. The third group of organelles consisted of rod-shaped bodies about 0.2-0.3 mum in diameter and 1-8 mum in length; these were usually stationary.3. All the round particles appeared to move independently of each other with a saltatory motion. The somatopetally and somatofugally travelling particles had statistically different mean velocities of 0.98 and 1.32 mum/sec respectively.4. Round particles often crossed the node of Ranvier with no appreciable change in velocity. Some, however, were temporarily arrested at the entrance to the node.5. While the rod-shaped organelles were usually stationary, they occasionally moved rapidly lengthwise for distances of up to 10 mum. Rarely a rod-shaped organelle exhibited a continuous saltatory motion.6. Round particles often travelled in either direction along the edge of rod-shaped organelles. One rod was observed to move along the path previously taken by a round particle.7. The findings are discussed with respect to (a) the normality of the preparations, (b) the numbers of particles travelling in each direction, (c) the nature of the organelles, and (d) the mechanisms underlying the motion.8. We suggest that particles move along microtubules which have specific directionalities and particle affinities. The microtubules are in bundles and are closely associated with rod-shaped mitochondria.
Berlinrood,
Patterns of particle movement in nerve fibres in vitro. An analysis by photokymography and microscopy.
1972, Pubmed,
Xenbase
Berlinrood,
Patterns of particle movement in nerve fibres in vitro. An analysis by photokymography and microscopy.
1972,
Pubmed
,
Xenbase Blume,
[Histological, histochemical and statistical investigations on length and distribution of mitochondria in segmented peripheral nerve fibers and their Schwann cells of the N. intercostalis in Bos taurus].
1964,
Pubmed Borisy,
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.
1967,
Pubmed Borisy,
The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus.
1967,
Pubmed Dahlström,
Effect of colchicine on transport of amine storage granules in sympathetic nerves of rat.
1968,
Pubmed Edstrom,
Fast axonal transport in vitro in the sciatic system of the frog.
1972,
Pubmed Edström,
Retrograde axonal transport of proteins in vitro in frog sciatic nerves.
1973,
Pubmed Freed,
The association of a class of saltatory movements with microtubules in cultured cells.
1970,
Pubmed Hökfelt,
Effects of two mitosis inhibitors (colchicine and vinblastine) on the distribution and axonal transport of noradrenaline storage particles, studied by fluorescence and electron microscopy.
1971,
Pubmed HUGHES,
The growth of embryonic neurites; a study of cultures of chick neural tissues.
1953,
Pubmed Hutchinson,
Conduction velocity in myelinated nerve fibres of Xenopus laevis.
1970,
Pubmed
,
Xenbase Huxley,
Proposed mechanism of force generation in striated muscle.
1971,
Pubmed James,
The effect of colchicine on the transport of axonal protein in the chicken.
1970,
Pubmed Johnson,
Accumulation of material at severed ends of myelinated nerve fibers.
1969,
Pubmed Kirkpatrick,
Axoplasmic flow in human sural nerve.
1973,
Pubmed Kirkpatrick,
Visualization of axoplasmic flow in vitro by Nomarski microscopy. Comparison to rapid flow of radioactive proteins.
1972,
Pubmed Kreutzberg,
Neuronal dynamics and axonal flow. IV. Blockage of intra-axonal enzyme transport by colchicine.
1969,
Pubmed Litchy,
Uptake and retrograde transport of horseradish peroxidase in frog sartorius nerve in vitro.
1973,
Pubmed LUBINSKA,
Outflow from cut ends of nerve fibres.
1956,
Pubmed Margulis,
Colchicine-sensitive microtubules.
1973,
Pubmed NAKAI,
Dissociated dorsal root ganglia in tissue culture.
1956,
Pubmed Norström,
Effects of colchicine on axonal transport and ultrastructure of the hypothalamo-neurohypophyseal system of the rat.
1971,
Pubmed Ochs,
Metabolic dependence of fast axoplasmic transport in nerve.
1970,
Pubmed Ochs,
Characteristics and a model for fast axoplasmic transport in nerve.
1971,
Pubmed Partlow,
Transport of axonal enzymes in surviving segments of frog sciatic nerve.
1972,
Pubmed Raine,
On the association between microtubules and mitochondria within axons.
1971,
Pubmed Rebhun,
Polarized intracellular particle transport: saltatory movements and cytoplasmic streaming.
1972,
Pubmed Smith,
Microtubule and neurofilament densities in amphibian spinal root nerve fibers: relationship to axoplasmic transport.
1973,
Pubmed
,
Xenbase Smith,
Detection of organelles in myelinated nerve fibers by dark-field microscopy.
1972,
Pubmed
,
Xenbase Smith,
Types of motor units in the skeletal muscle of Xenopus laevis.
1968,
Pubmed
,
Xenbase Wuerker,
Neuronal microtubules, neurofilaments, and microfilaments.
1972,
Pubmed Zenker,
A-alpha-nerve-fiber: number of neurotubules in the stem fibre and in the terminal branches.
1973,
Pubmed
