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XB-ART-2954
Pflugers Arch 2004 Dec 01;4493:271-7. doi: 10.1007/s00424-004-1324-z.
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Novel outwardly rectifying anion conductance in Xenopus oocytes.

Reyes JP, Hernandez-Carballo CY, Pérez-Cornejo P, Meza U, Espinosa-Tanguma R, Arreola J.


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We describe a novel, strongly outwardly rectifying anion current in Xenopus laevis oocytes, that we have named I(Cl,Or)- The properties of I(Cl,Or) are different from those of any other anion conductance previously described in these cells. Typically, I(Cl,Or) amplitude was small when extracellular Cl- (Cle) was the permeant anion. However, when Cle was replaced by lyotropic anions I(Cl,Or) became evident as a time-independent current. (ICl,Or) was voltage dependent and showed a remarkable outwards rectification with little or no inwards tail current. The relative selectivity sequence determined from current amplitudes was: SCN- > or = ClO4- > I- > Br- > or = NO3- > Cl- x I(Cl,Or) was insensitive to Gd3+ but was blocked by micromolar concentrations of niflumic acid, DIDS or Zn2+. Furthermore, I(Cl,Or) was not affected by buffering intracellular Ca2+ with BAPTA. Low extracellular pH inhibited I(Cl,Or) with a pK of 5.8. We propose that I(Cl,Or) might result from activation of endogenous ClC-5-like Cl- channels present in Xenopus oocytes.

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Species referenced: Xenopus laevis
Genes referenced: clcn5 nbl1 sri uqcc6

References [+] :
Ackerman, Hypotonicity activates a native chloride current in Xenopus oocytes. 1994, Pubmed, Xenbase