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Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.
Carroll D, Wright SH, Wolff RK, Grzesiuk E, Maryon EB.
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When DNA molecules are injected into Xenopus oocyte nuclei, they can recombine with each other. With bacteriophage lambda DNAs, it was shown that this recombination is stimulated greatly by introduction of double-strand breaks into the substrates and is dependent on homologous overlaps in the recombination interval. With plasmid DNAs it was shown that little or no recombination occurs between circular molecules but both intra- and intermolecular events take place very efficiently with linear molecules. As with the lambda substrates, homology was required to support recombination; no simple joining of ends was observed. Blockage of DNA ends with nonhomologous sequences interfered with recombination, indicating that ends are used directly to initiate homologous interactions. These observations are combined to evaluate possible models of recombination in the oocytes. Because each oocyte is capable of recombining nanogram quantities of linear DNA, this system offers exceptional opportunities for detailed molecular analysis of the recombination process in a higher organism.
Baker,
The genetic control of meiosis.
1976, Pubmed
Baker,
The genetic control of meiosis.
1976,
Pubmed Benbow,
Recombinant DNA formation in a cell-free system from Xenopus laevis eggs.
1977,
Pubmed
,
Xenbase Bendig,
Persistence and expression of histone genes injected into Xenopus eggs in early development.
1981,
Pubmed
,
Xenbase Boyer,
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
1969,
Pubmed Brenner,
Effect of insertions, deletions, and double-strand breaks on homologous recombination in mouse L cells.
1985,
Pubmed Carroll,
Genetic recombination of Xenopus laevis 5 S DNA in bacteria.
1984,
Pubmed
,
Xenbase Carroll,
Genetic recombination of bacteriophage lambda DNAs in Xenopus oocytes.
1983,
Pubmed
,
Xenbase Chakrabarti,
Recombination and deletion of sequences in shuttle vector plasmids in mammalian cells.
1985,
Pubmed DasGupta,
Concerted strand exchange and formation of Holliday structures by E. coli RecA protein.
1981,
Pubmed de Saint Vincent,
Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.
1983,
Pubmed Doherty,
Genetic recombination of bacterial plasmid DNA. Physical and genetic analysis of the products of plasmid recombination in Escherichia coli.
1983,
Pubmed Dressler,
Molecular mechanisms in genetic recombination.
1982,
Pubmed Folger,
Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.
1982,
Pubmed Folger,
Analysis of homologous recombination in cultured mammalian cells.
1984,
Pubmed Gandini Attardi,
Formation of branched DNA structures by Xenopus laevis oocyte extract.
,
Pubmed
,
Xenbase Gargiulo,
Analysis of the chromatin assembled in germinal vesicles of Xenopus oocytes.
1983,
Pubmed
,
Xenbase Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed Hall,
Mechanisms of DNA replication and mutagenesis in ultraviolet-irradiated bacteria and mammalian cells.
1981,
Pubmed Hanahan,
Studies on transformation of Escherichia coli with plasmids.
1983,
Pubmed Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase Kucherlapati,
Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.
1984,
Pubmed Kunz,
Phenomenology and genetic control of mitotic recombination in yeast.
1981,
Pubmed Lin,
Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences.
1985,
Pubmed Lin,
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
1984,
Pubmed Maniatis,
Targeting in mammalian cells.
,
Pubmed Mertz,
Linear DNA does not form chromatin containing regularly spaced nucleosomes.
1982,
Pubmed
,
Xenbase Mertz,
In vivo catenation and decatenation of DNA.
1983,
Pubmed
,
Xenbase Miller,
Template structural requirements for transcription in vivo by RNA polymerase II.
1982,
Pubmed
,
Xenbase Miller,
High-efficiency ligation and recombination of DNA fragments by vertebrate cells.
1983,
Pubmed Morrison,
Transformation and preservation of competent bacterial cells by freezing.
1979,
Pubmed Orr-Weaver,
Yeast transformation: a model system for the study of recombination.
1981,
Pubmed Perucho,
Genetic and physical linkage of exogenous sequences in transformed cells.
1980,
Pubmed Radding,
Homologous pairing and strand exchange in genetic recombination.
1982,
Pubmed Roth,
Relative rates of homologous and nonhomologous recombination in transfected DNA.
1985,
Pubmed Rubnitz,
Rapid assay for extrachromosomal homologous recombination in monkey cells.
1985,
Pubmed Rusconi,
Transformation of frog embryos with a rabbit beta-globin gene.
1981,
Pubmed
,
Xenbase Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase Shapira,
Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.
1983,
Pubmed Small,
Recombination during gene transfer into mouse cells can restore the function of deleted genes.
1983,
Pubmed Smith,
Homologous recombination between defective neo genes in mouse 3T6 cells.
1984,
Pubmed Smithies,
Homologous recombination with DNA introduced into mammalian cells.
1984,
Pubmed Smithies,
Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination.
,
Pubmed Stahl,
In phage lambda, cos is a recombinator in the red pathway.
1985,
Pubmed Subramani,
Homologous and nonhomologous recombination in monkey cells.
1983,
Pubmed Symington,
Intramolecular recombination of linear DNA catalyzed by the Escherichia coli RecE recombination system.
1985,
Pubmed Szostak,
The double-strand-break repair model for recombination.
1983,
Pubmed Upcroft,
Analysis of recombination in mammalian cells using SV40 genome segments having homologous overlapping termini.
1980,
Pubmed Wake,
How damaged is the biologically active subpopulation of transfected DNA?
1984,
Pubmed Wake,
Simian virus 40 recombinants are produced at high frequency during infection with genetically mixed oligomeric DNA.
1979,
Pubmed Wake,
Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.
1985,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase Wilson,
Somatic cells efficiently join unrelated DNA segments end-to-end.
1982,
Pubmed Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
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