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Sequence and developmental expression of mRNA coding for a gap junction protein in Xenopus.
Gimlich RL, Kumar NM, Gilula NB.
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Cloned complementary DNAs representing the complete coding sequence for an embryonic gap junction protein in the frog Xenopus laevis have been isolated and sequenced. The cDNAs hybridize with an RNA of 1.5 kb that is first detected in gastrulating embryos and accumulates throughout gastrulation and neurulation. By the tailbud stage, the highest abundance of the transcript is found in the region containing ventroposterior endoderm and the rudiment of the liver. In the adult, transcripts are present in the lungs, alimentary tract organs, and kidneys, but are not detected in the brain, heart, body wall and skeletal muscles, spleen, or ovary. The gene encoding this embryonic gap junction protein is present in only one or a few copies in the frog genome. In vitro translation of RNA synthesized from the cDNA template produces a 30-kD protein, as predicted by the coding sequence. This product has extensive sequence similarity to mammalian gap junction proteins in its putative transmembrane and extracellular domains, but has diverged substantially in two of its intracellular domains.
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980, Pubmed
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed Beyer,
Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
1987,
Pubmed Biggin,
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
1983,
Pubmed Blackshaw,
Low resistance junctions between mesoderm cells during development of trunk muscles.
1976,
Pubmed
,
Xenbase Brink,
Evidence for fixed charge in the nexus.
1980,
Pubmed Burke,
High-sensitivity S1 mapping with single-stranded [32P]DNA probes synthesized from bacteriophage M13mp templates.
1984,
Pubmed Chase,
Biogenesis of mitochondria during Xenopus laevis development.
1972,
Pubmed
,
Xenbase Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Dreyfuss,
Physical change in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription.
1984,
Pubmed Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Dworkin,
Construction of a cloned library of expressed embryonic gene sequences from Xenopus laevis.
1980,
Pubmed
,
Xenbase Epstein,
A study of communication specificity between cells in culture.
1977,
Pubmed Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed Flagg-Newton,
Permeability of the cell-to-cell membrane channels in mammalian cell juncton.
1979,
Pubmed Fraser,
Selective disruption of gap junctional communication interferes with a patterning process in hydra.
1987,
Pubmed Gilula,
Metabolic coupling, ionic coupling and cell contacts.
1972,
Pubmed Gimlich,
Improved fluorescent compounds for tracing cell lineage.
1985,
Pubmed
,
Xenbase Glisin,
Ribonucleic acid isolated by cesium chloride centrifugation.
1974,
Pubmed Gros,
Comparative analysis of the gap junction protein from rat heart and liver: is there a tissue specificity of gap junctions?
1983,
Pubmed Guthrie,
Patterns of junctional communication in the early amphibian embryo.
,
Pubmed
,
Xenbase Hertzberg,
A protein homologous to the 27,000 dalton liver gap junction protein is present in a wide variety of species and tissues.
1984,
Pubmed Hertzberg,
Comparative analysis of the major polypeptides from liver gap junctions and lens fiber junctions.
1982,
Pubmed Hirose,
Clonal organization of the central nervous system of the frog. I. Clones stemming from individual blastomeres of the 16-cell and earlier stages.
1979,
Pubmed
,
Xenbase Johnson,
A lens intercellular junction protein, MP26, is a phosphoprotein.
1986,
Pubmed Kistler,
Homologies between gap junction proteins in lens, heart and liver.
1988,
Pubmed Krebs,
Phosphorylation-dephosphorylation of enzymes.
1979,
Pubmed Kumar,
Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.
1986,
Pubmed Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed Lawrence,
Transmission of hormonal stimulation by cell-to-cell communication.
1978,
Pubmed Lev,
Developmental expression of two cloned sequences coding for rare sea urchin embryo messages.
1980,
Pubmed Loewenstein,
Junctional intercellular communication: the cell-to-cell membrane channel.
1981,
Pubmed Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Messing,
New M13 vectors for cloning.
1983,
Pubmed Michalke,
Communication between cells of different type.
1971,
Pubmed Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase Murray,
Hormone-induced intercellular signal transfer dissociates cyclic AMP-dependent protein kinase.
1984,
Pubmed Neyton,
Single-channel currents of an intercellular junction.
,
Pubmed Nicholson,
Two homologous protein components of hepatic gap junctions.
,
Pubmed Patschinsky,
Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
1982,
Pubmed Paul,
Molecular cloning of cDNA for rat liver gap junction protein.
1986,
Pubmed Pitts,
Permeability of junctions between animal cells. Intercellular transfer of nucleotides but not of macromolecules.
1977,
Pubmed Saez,
cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide.
1986,
Pubmed Sagata,
A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis.
1980,
Pubmed
,
Xenbase Sargent,
Cell interactions and the control of gene activity during early development of Xenopus laevis.
1986,
Pubmed
,
Xenbase Scheller,
Specific representation of cloned repetitive DNA sequences in sea urchin RNAs.
1978,
Pubmed Schwarzmann,
Diameter of the cell-to-cell junctional membrane channels as probed with neutral molecules.
1981,
Pubmed Simpson,
Size limit of molecules permeating the junctional membrane channels.
1977,
Pubmed Spray,
Physiology and pharmacology of gap junctions.
1985,
Pubmed Unwin,
Is there a common design for cell membrane channels?
,
Pubmed Warner,
The electrical properties of the ectoderm in the amphibian embryo during induction and early development of the nervous system.
1973,
Pubmed Warner,
Antibodies to gap-junctional protein selectively disrupt junctional communication in the early amphibian embryo.
,
Pubmed
,
Xenbase Young,
Functional assembly of gap junction conductance in lipid bilayers: demonstration that the major 27 kd protein forms the junctional channel.
1987,
Pubmed Zimmer,
Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures.
1987,
Pubmed
