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Homologous recombination of DNA molecules injected into Xenopus laevis oocyte nuclei is extremely efficient when those molecules are linear and have overlapping homologous ends. It was previously shown that a 5'----3' exonuclease activity in oocytes attacks injected linear DNAs and leaves them with single-stranded 3' tails. We tested the hypothesis that such tailed molecules are early intermediates on the pathway to recombination products. Substrates with 3' tails were made in vitro and injected into oocytes, where they recombined rapidly and efficiently. In experiments with mixed substrates, molecules with 3' tails entered recombination intermediates and products more rapidly than did molecules with flush ends. Molecules endowed in vitro with 5' tails also recombined efficiently in oocytes, but their rate was not faster than for flush-ended substrates. In most cases, the 5' tails served as templates for resynthesis of the 3' strands, regenerating duplex ends which then entered the normal recombination pathway. In oocytes from one animal, some of the 5' tails were removed, and this was exacerbated when resynthesis was partially blocked. Analysis by two-dimensional gel electrophoresis of recombination intermediates from 5'-tailed substrates confirmed that they had acquired 3' tails as a result of the action of the 5'----3' exonuclease. These results demonstrate that homologous recombination in oocytes proceeds via a pathway that involves single-stranded 3' tails. Molecular models incorporating this feature are discussed.
Anderson,
Recombination of homologous DNA fragments transfected into mammalian cells occurs predominantly by terminal pairing.
1986, Pubmed
Anderson,
Recombination of homologous DNA fragments transfected into mammalian cells occurs predominantly by terminal pairing.
1986,
Pubmed Baur,
Intermolecular homologous recombination in plants.
1990,
Pubmed Cao,
A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae.
1990,
Pubmed Carroll,
Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.
1986,
Pubmed
,
Xenbase Carter,
The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease.
1971,
Pubmed Cassuto,
Role of exonuclease and beta protein of phage lambda in genetic recombination. V. Recombination of lambda DNA in vitro.
1971,
Pubmed Cassuto,
Mechanism for the action of lambda exonuclease in genetic recombination.
1971,
Pubmed Church,
Genomic sequencing.
1984,
Pubmed Cox,
Enzymes of general recombination.
1987,
Pubmed Craigie,
Mechanism of transposition of bacteriophage Mu: structure of a transposition intermediate.
1985,
Pubmed Doherty,
Genetic recombination of bacterial plasmid DNA. Physical and genetic analysis of the products of plasmid recombination in Escherichia coli.
1983,
Pubmed Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed Formosa,
DNA synthesis dependent on genetic recombination: characterization of a reaction catalyzed by purified bacteriophage T4 proteins.
1986,
Pubmed Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase Haber,
Physical monitoring of meiotic and mitotic recombination in yeast.
1988,
Pubmed Hsieh,
Formation of joint DNA molecules by two eukaryotic strand exchange proteins does not require melting of a DNA duplex.
1989,
Pubmed Klar,
Initiation of meiotic recombination by double-strand DNA breaks in S. pombe.
1986,
Pubmed Kolodkin,
Double-strand breaks can initiate meiotic recombination in S. cerevisiae.
1986,
Pubmed Legerski,
Removal of 2',3'-dideoxynucleotide residues from injected DNA in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase Lichten,
Detection of heteroduplex DNA molecules among the products of Saccharomyces cerevisiae meiosis.
1990,
Pubmed Lin,
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
1984,
Pubmed Lindahl,
Deoxyribonuclease IV: a new exonuclease from mammalian tissues.
1969,
Pubmed Lindahl,
The action pattern of mammalian deoxyribonuclease IV.
1971,
Pubmed Maryon,
Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: evidence for a nonconservative mechanism of homologous recombination.
1991,
Pubmed
,
Xenbase Maryon,
Degradation of linear DNA by a strand-specific exonuclease activity in Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase Meselson,
A general model for genetic recombination.
1975,
Pubmed Mosig,
The essential role of recombination in phage T4 growth.
1987,
Pubmed Resnick,
The repair of double-strand breaks in DNA; a model involving recombination.
1976,
Pubmed Rudin,
Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae.
1989,
Pubmed Seidman,
Intermolecular homologous recombination between transfected sequences in mammalian cells is primarily nonconservative.
1987,
Pubmed Smith,
Roles of RecBC enzyme and chi sites in homologous recombination.
1984,
Pubmed Stahl,
Roles of double-strand breaks in generalized genetic recombination.
1986,
Pubmed Sun,
Double-strand breaks at an initiation site for meiotic gene conversion.
1989,
Pubmed Symington,
Intramolecular recombination of linear DNA catalyzed by the Escherichia coli RecE recombination system.
1985,
Pubmed Szostak,
The double-strand-break repair model for recombination.
1983,
Pubmed Taylor,
Unwinding and rewinding of DNA by the RecBC enzyme.
1980,
Pubmed Thaler,
DNA double-chain breaks in recombination of phage lambda and of yeast.
1988,
Pubmed Thaler,
Double-chain-cut sites are recombination hotspots in the Red pathway of phage lambda.
1987,
Pubmed Wake,
Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.
1985,
Pubmed West,
Processing of recombination intermediates in vitro.
1990,
Pubmed White,
Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.
1990,
Pubmed Wu,
Formation of nascent heteroduplex structures by RecA protein and DNA.
1982,
Pubmed
