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Proc Natl Acad Sci U S A
2005 Feb 01;1025:1329-32. doi: 10.1073/pnas.0409413102.
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Chromatin remodeling by DNA bending, not twisting.
Lorch Y, Davis B, Kornberg RD.
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Single-stranded regions (gaps) in nucleosomal DNA interfere with action of the RSC chromatin-remodeling complex, monitored by exposure of restriction endonuclease cutting sites. Single-strand breaks (nicks) in the DNA, by contrast, have no effect. Gaps on one side of the cutting site are inhibitory, but gaps on the other side are not. A gap >100 bp from the cutting site is as effective as a gap <20 bp from the site. These findings suggest a remodeling process involving bending, but not twisting, of the DNA and further point to the propagation of a bent region (loop or bulge) from one end of the nucleosome to the other.
Aoyagi,
hSWI/SNF-catalyzed nucleosome sliding does not occur solely via a twist-diffusion mechanism.
2002, Pubmed,
Xenbase
Aoyagi,
hSWI/SNF-catalyzed nucleosome sliding does not occur solely via a twist-diffusion mechanism.
2002,
Pubmed
,
Xenbase Cairns,
RSC, an essential, abundant chromatin-remodeling complex.
1996,
Pubmed Cloutier,
Spontaneous sharp bending of double-stranded DNA.
2004,
Pubmed Côté,
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
1994,
Pubmed Havas,
Generation of superhelical torsion by ATP-dependent chromatin remodeling activities.
2000,
Pubmed
,
Xenbase Imbalzano,
Facilitated binding of TATA-binding protein to nucleosomal DNA.
1994,
Pubmed Kassabov,
SWI/SNF unwraps, slides, and rewraps the nucleosome.
2003,
Pubmed
,
Xenbase Längst,
ISWI induces nucleosome sliding on nicked DNA.
2001,
Pubmed Längst,
Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.
1999,
Pubmed Lorch,
RSC unravels the nucleosome.
2001,
Pubmed
,
Xenbase Lorch,
Isolation and assay of the RSC chromatin-remodeling complex from Saccharomyces cerevisiae.
2004,
Pubmed Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed Lorch,
Activated RSC-nucleosome complex and persistently altered form of the nucleosome.
1998,
Pubmed Lorch,
Histone octamer transfer by a chromatin-remodeling complex.
1999,
Pubmed
,
Xenbase Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed Polach,
Mechanism of protein access to specific DNA sequences in chromatin: a dynamic equilibrium model for gene regulation.
1995,
Pubmed Prunell,
Relation of nucleosomes to DNA sequences.
1978,
Pubmed Saha,
Chromatin remodeling by RSC involves ATP-dependent DNA translocation.
2002,
Pubmed Schiessel,
Polymer reptation and nucleosome repositioning.
2001,
Pubmed Studitsky,
A histone octamer can step around a transcribing polymerase without leaving the template.
1994,
Pubmed
