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We used quick-freeze, deep-etch, rotary replication and immunogold cytochemistry to identify a new structure at focal contacts. In Xenopus fibroblasts, elongated aggregates of particles project from the membrane to contact bundles of actin microfilaments. Before terminating, a single bundle of microfilaments interacts with several aggregates that appear intermittently over a distance of several microns. Aggregates are enriched in proteins believed to mediate actin-membrane interactions at focal contacts, including beta 1-integrin, vinculin, and talin, but they appear to contain less alpha-actinin and filamin. We also identified a second, smaller class of aggregates of membrane particles that contained beta 1-integrin but not vinculin or talin and that were not associated with actin microfilaments. Our results indicate that vinculin, talin, and beta 1-integrin are assembled into distinctive structures that mediate multiple lateral interactions between microfilaments and the membrane at focal contacts.
Abercrombie,
The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
1971, Pubmed
Abercrombie,
The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
1971,
Pubmed Albelda,
Integrins and other cell adhesion molecules.
1990,
Pubmed Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase Avnur,
Substrate-attached membranes of cultured cells isolation and characterization of ventral cell membranes and the associated cytoskeleton.
1981,
Pubmed Beckerle,
Identification of a new protein localized at sites of cell-substrate adhesion.
1986,
Pubmed Bloch,
Cytoskeletal components of the vertebrate neuromuscular junction: vinculin, alpha-actinin, and filamin.
1983,
Pubmed
,
Xenbase Bloch,
Microfilaments and actin-associated proteins at sites of membrane-substrate attachment within acetylcholine receptor clusters.
1989,
Pubmed Brands,
Disintegration of adhesion plaques in chicken embryo fibroblasts upon Rous sarcoma virus-induced transformation: different dissociation rates for talin and vinculin.
1990,
Pubmed Bretscher,
Fimbrin, a new microfilament-associated protein present in microvilli and other cell surface structures.
1980,
Pubmed Bridgman,
The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance.
1984,
Pubmed
,
Xenbase Brown,
Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins.
1990,
Pubmed Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed Burridge,
A new protein of adhesion plaques and ruffling membranes.
1983,
Pubmed Burridge,
An interaction between vinculin and talin.
,
Pubmed Burridge,
Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
1988,
Pubmed Chen,
Development of cell surface linkage complexes in cultured fibroblasts.
1985,
Pubmed Chen,
Immunoelectron microscopic studies of the sites of cell-substratum and cell-cell contacts in cultured fibroblasts.
1982,
Pubmed Chia,
The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly.
1993,
Pubmed Cohen,
Clusters of intramembrane particles associated with binding sites for alpha-bungarotoxin in cultured chick myotubes.
1979,
Pubmed Coutu,
cDNA-derived sequence of chicken embryo vinculin.
1988,
Pubmed Damsky,
Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture.
1985,
Pubmed Dejana,
Fibronectin and vitronectin regulate the organization of their respective Arg-Gly-Asp adhesion receptors in cultured human endothelial cells.
1988,
Pubmed Duband,
Fibronectin receptor exhibits high lateral mobility in embryonic locomoting cells but is immobile in focal contacts and fibrillar streaks in stationary cells.
1988,
Pubmed Feltkamp,
Organization of talin and vinculin in adhesion plaques of wet-cleaved chicken embryo fibroblasts.
1991,
Pubmed Geiger,
Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes.
1980,
Pubmed Geiger,
Restricted mobility of membrane constituents in cell-substrate focal contacts of chicken fibroblasts.
1982,
Pubmed Geiger,
A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.
1979,
Pubmed Gorlin,
Human endothelial actin-binding protein (ABP-280, nonmuscle filamin): a molecular leaf spring.
1990,
Pubmed Heath,
Cell to substratum contacts of chick fibroblasts and their relation to the microfilament system. A correlated interference-reflexion and high-voltage electron-microscope study.
1978,
Pubmed Heuser,
Filament organization revealed in platinum replicas of freeze-dried cytoskeletons.
1980,
Pubmed Hirokawa,
Interactions between actin filaments and between actin filaments and membranes in quick-frozen and deeply etched hair cells of the chick ear.
1982,
Pubmed Horwitz,
Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage.
,
Pubmed Imamura,
Substructure and higher structure of chicken smooth muscle alpha-actinin molecule.
1988,
Pubmed Juliano,
Signal transduction from the extracellular matrix.
1993,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Luther,
Formaldehyde-amine fixatives for immunocytochemistry of cultured Xenopus myocytes.
1989,
Pubmed
,
Xenbase Maher,
An integral membrane protein antigen associated with the membrane attachment sites of actin microfilaments is identified as an integrin beta-chain.
1988,
Pubmed Marcantonio,
Antibodies to the conserved cytoplasmic domain of the integrin beta 1 subunit react with proteins in vertebrates, invertebrates, and fungi.
1988,
Pubmed Maupin,
Improved preservation and staining of HeLa cell actin filaments, clathrin-coated membranes, and other cytoplasmic structures by tannic acid-glutaraldehyde-saponin fixation.
1983,
Pubmed Molony,
Molecular shape and self-association of vinculin and metavinculin.
1985,
Pubmed Muguruma,
Direct interactions between talin and actin.
1990,
Pubmed Neff,
A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
1982,
Pubmed Nermut,
Cell/substratum adhesions in RSV-transformed rat fibroblasts.
1991,
Pubmed Nermut,
Electron microscopy and structural model of human fibronectin receptor.
1988,
Pubmed Nicol,
Labeling of structural elements at the ventral plasma membrane of fibroblasts with the immunogold technique.
1987,
Pubmed Niggli,
Interaction in situ of the cytoskeletal protein vinculin with bilayers studied by introducing a photoactivatable fatty acid into living chicken embryo fibroblasts.
1990,
Pubmed Otey,
An interaction between alpha-actinin and the beta 1 integrin subunit in vitro.
1990,
Pubmed Pavalko,
Disruption of the actin cytoskeleton after microinjection of proteolytic fragments of alpha-actinin.
1991,
Pubmed Pumplin,
Acetylcholine receptor clusters of rat myotubes have at least three domains with distinctive cytoskeletal and membranous components.
1989,
Pubmed Pumplin,
Quick-freeze, deep-etch replication of cells in monolayers.
1990,
Pubmed
,
Xenbase Regen,
Dynamics of beta 1 integrin-mediated adhesive contacts in motile fibroblasts.
1992,
Pubmed Rothberg,
Caveolin, a protein component of caveolae membrane coats.
1992,
Pubmed Ruhnau,
Evidence for direct binding of vinculin to actin filaments.
1988,
Pubmed Ruoslahti,
Fibronectin and its receptors.
1988,
Pubmed Saga,
Expression of meta-vinculin associated with differentiation of chicken embryonal muscle cells.
1985,
Pubmed
,
Xenbase Schliwa,
Structural interaction of cytoskeletal components.
1981,
Pubmed Semich,
Organization of the cytoskeleton and the focal contacts of bovine aortic endothelial cells cultured on type I and III collagen.
1990,
Pubmed Singer,
Scanning electron microscopy of focal contacts on the substratum attachment surface of fibroblasts adherent to fibronectin.
1989,
Pubmed Singer,
The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts.
1979,
Pubmed St-Onge,
Research on the mechanism of interaction between actin and membrane lipids.
1990,
Pubmed Turner,
Paxillin: a new vinculin-binding protein present in focal adhesions.
1990,
Pubmed Wang,
Filamin, a new high-molecular-weight protein found in smooth muscle and non-muscle cells.
1975,
Pubmed Wildhaber,
The control of freeze-drying with deuterium oxide (D2O).
1982,
Pubmed Wilkins,
A re-examination of the interaction of vinculin with actin.
1986,
Pubmed Wuestehube,
F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes.
1987,
Pubmed
