Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Cytoplasmic regulation of the length of poly(A) on mRNA is a well-characterized process involved in translational control during development. In contrast, there is no direct in vivo evidence for regulation of the length of poly(A) added during nuclear pre-mRNA processing in somatic cells. We previously reported that Xenopus serum albumin [Schoenberg et al. (1989) Mol. Endocrinol. 3, 805-815] and transferrin [Pastori et al. (1992) J. Steroid Biochem. Mol. Biol. 42, 649-657], mRNA have exceptionally short poly(A) tails ranging from 12 to 17 residues, whereas vitellogenin mRNA has long poly(A). An RT-PCR protocol was adapted to determine the length of poly(A) added onto pre-mRNA, defined here as that species bearing the terminal intron. Using this assay we show that vitellogenin pre-mRNA has the same long poly(A) tail as mature vitellogenin mRNA. In contrast, albumin pre-mRNA has the same short poly(A) as found on fully-processed albumin mRNA. These results indicate that the short poly(A) tail on albumin mRNA results from regulation of poly(A) addition during nuclear 3' processing.
???displayArticle.pubmedLink???
8918815 ???displayArticle.pmcLink???PMC146192 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Baurén,
Splicing of Balbiani ring 1 gene pre-mRNA occurs simultaneously with transcription.
1994, Pubmed
Baurén,
Splicing of Balbiani ring 1 gene pre-mRNA occurs simultaneously with transcription.
1994,
Pubmed Bienroth,
Assembly of a processive messenger RNA polyadenylation complex.
1993,
Pubmed Carrazana,
The vasopressin mRNA poly(A) tract is unusually long and increases during stimulation of vasopressin gene expression in vivo.
1988,
Pubmed Decker,
A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation.
1993,
Pubmed Dompenciel,
Purification and characterization of an estrogen-regulated Xenopus liver polysomal nuclease involved in the selective destabilization of albumin mRNA.
1995,
Pubmed
,
Xenbase Greenberg,
Deadenylylation: a mechanism controlling c-fos mRNA decay.
1990,
Pubmed Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed Krizman,
Efficient selection of 3'-terminal exons from vertebrate DNA.
1993,
Pubmed MacDonald,
The 64-kilodalton subunit of the CstF polyadenylation factor binds to pre-mRNAs downstream of the cleavage site and influences cleavage site location.
1994,
Pubmed Manley,
A complex protein assembly catalyzes polyadenylation of mRNA precursors.
1995,
Pubmed Moskaitis,
Xenopus laevis serum albumin: sequence of the complementary deoxyribonucleic acids encoding the 68- and 74-kilodalton peptides and the regulation of albumin gene expression by thyroid hormone during development.
1989,
Pubmed
,
Xenbase Muhlrad,
Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.
1994,
Pubmed Murthy,
Characterization of the multisubunit cleavage-polyadenylation specificity factor from calf thymus.
1992,
Pubmed Nesic,
Lack of an effect of the efficiency of RNA 3'-end formation on the efficiency of removal of either the final or the penultimate intron in intact cells.
1995,
Pubmed Pastori,
Estrogen-induced ribonuclease activity in Xenopus liver.
1991,
Pubmed
,
Xenbase Pastori,
Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis.
1991,
Pubmed
,
Xenbase Pastori,
Differential regulation and polyadenylation of transferrin mRNA in Xenopus liver and oviduct.
1992,
Pubmed
,
Xenbase Riegel,
Transcriptional and post-transcriptional inhibition of albumin gene expression by estrogen in Xenopus liver.
1986,
Pubmed
,
Xenbase Sachs,
Nuclear polyadenylate-binding protein.
1985,
Pubmed Sachs,
Poly(A) tail metabolism and function in eucaryotes.
1993,
Pubmed Sallés,
Rapid and sensitive analysis of mRNA polyadenylation states by PCR.
1995,
Pubmed
,
Xenbase Schoenberg,
Extranuclear estrogen-regulated destabilization of Xenopus laevis serum albumin mRNA.
1989,
Pubmed
,
Xenbase Sheets,
Two phases in the addition of a poly(A) tail.
1989,
Pubmed Sheflin,
Assessment of transcript polyadenylation by 3' RACE: the response of epidermal growth factor messenger ribonucleic acid to thyroid hormone in the thyroid and submaxillary glands.
1995,
Pubmed Shyu,
Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
1991,
Pubmed Takagaki,
The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs.
1992,
Pubmed Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase Wahle,
A novel poly(A)-binding protein acts as a specificity factor in the second phase of messenger RNA polyadenylation.
1991,
Pubmed Wahle,
The biochemistry of 3'-end cleavage and polyadenylation of messenger RNA precursors.
1992,
Pubmed Wahle,
Poly(A) tail length control is caused by termination of processive synthesis.
1995,
Pubmed Wilson,
Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
1988,
Pubmed Wormington,
Overexpression of poly(A) binding protein prevents maturation-specific deadenylation and translational inactivation in Xenopus oocytes.
1996,
Pubmed
,
Xenbase Zingg,
Regulation of poly(A) tail size of vasopressin mRNA.
1988,
Pubmed
