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Scaffold-associated regions (SARs) were studied in Drosophila melanogaster by expressing a synthetic, high-affinity SAR-binding protein called MATH (multi-AT-hook), which consists of reiterated AT-hook peptide motifs; each motif is known to recognize a wide variety of short AT-rich sequences. MATH proteins were expressed specifically in the larval eye imaginal discs by means of the tetracycline-regulated transactivation system and tested for their effect on position effect variegation (PEV). MATH20, a highly potent SAR ligand consisting of 20 AT-hooks, was found to suppress whitemottled 4 variegation. This suppression required MATH20 expression at an early larval developmental stage. Our data suggest an involvement of the high AT-rich SARs in higher order chromatin structure and gene expression.
Bode,
Biological significance of unwinding capability of nuclear matrix-associating DNAs.
1992, Pubmed
Bode,
Biological significance of unwinding capability of nuclear matrix-associating DNAs.
1992,
Pubmed Cockerill,
Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites.
1986,
Pubmed Dernburg,
Perturbation of nuclear architecture by long-distance chromosome interactions.
1996,
Pubmed Elgin,
Heterochromatin and gene regulation in Drosophila.
1996,
Pubmed Gasser,
Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster.
1986,
Pubmed Gossen,
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
1992,
Pubmed Hsieh,
Sequence and sequence variation within the 1.688 g/cm3 satellite DNA of Drosophila melanogaster.
1979,
Pubmed Jenuwein,
Extension of chromatin accessibility by nuclear matrix attachment regions.
1997,
Pubmed Karpen,
Position-effect variegation and the new biology of heterochromatin.
1994,
Pubmed Käs,
A model for chromatin opening: stimulation of topoisomerase II and restriction enzyme cleavage of chromatin by distamycin.
1993,
Pubmed Käs,
In vivo topoisomerase II cleavage of the Drosophila histone and satellite III repeats: DNA sequence and structural characteristics.
1992,
Pubmed Kirillov,
A role for nuclear NF-kappaB in B-cell-specific demethylation of the Igkappa locus.
1996,
Pubmed Klehr,
Scaffold-attached regions from the human interferon beta domain can be used to enhance the stable expression of genes under the control of various promoters.
1991,
Pubmed Laemmli,
Scaffold-associated regions: cis-acting determinants of chromatin structural loops and functional domains.
1992,
Pubmed Locke,
Dosage-dependent modifiers of position effect variegation in Drosophila and a mass action model that explains their effect.
1988,
Pubmed Mirkovitch,
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.
1984,
Pubmed Patton,
Position-independent germline transformation in Drosophila using a cuticle pigmentation gene as a selectable marker.
1992,
Pubmed Poljak,
SARs stimulate but do not confer position independent gene expression.
1994,
Pubmed Quiring,
Homology of the eyeless gene of Drosophila to the Small eye gene in mice and Aniridia in humans.
1994,
Pubmed Rattner,
Topoisomerase II alpha is associated with the mammalian centromere in a cell cycle- and species-specific manner and is required for proper centromere/kinetochore structure.
1996,
Pubmed Reeves,
The A.T-DNA-binding domain of mammalian high mobility group I chromosomal proteins. A novel peptide motif for recognizing DNA structure.
1990,
Pubmed Rubin,
Genetic transformation of Drosophila with transposable element vectors.
1982,
Pubmed Rubin,
Vectors for P element-mediated gene transfer in Drosophila.
1983,
Pubmed Spradling,
Transposition of cloned P elements into Drosophila germ line chromosomes.
1982,
Pubmed Strick,
SARs are cis DNA elements of chromosome dynamics: synthesis of a SAR repressor protein.
1995,
Pubmed
,
Xenbase Zhao,
SAR-dependent mobilization of histone H1 by HMG-I/Y in vitro: HMG-I/Y is enriched in H1-depleted chromatin.
1993,
Pubmed
